Protein Info for PP_2010 in Pseudomonas putida KT2440

Annotation: Cytochrome b561

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 181 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 51 to 69 (19 residues), see Phobius details amino acids 89 to 112 (24 residues), see Phobius details amino acids 149 to 168 (20 residues), see Phobius details PF01292: Ni_hydr_CYTB" amino acids 9 to 179 (171 residues), 92.6 bits, see alignment E=1.3e-30

Best Hits

Swiss-Prot: 46% identical to C561_ECOLI: Cytochrome b561 (cybB) from Escherichia coli (strain K12)

KEGG orthology group: K12262, cytochrome b561 (inferred from 100% identity to ppu:PP_2010)

MetaCyc: 46% identical to superoxide oxidase (Escherichia coli K-12 substr. MG1655)
RXN-20148 [EC: 1.10.3.17]

Predicted SEED Role

"Cytochrome B561"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.10.3.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LC5 at UniProt or InterPro

Protein Sequence (181 amino acids)

>PP_2010 Cytochrome b561 (Pseudomonas putida KT2440)
MVSSPPTTHYARLSIALHWLMLVLLAAVYALIELRGLFPKDSAERNLMKDLHFMLGLSVF
VLVWLRLAMRLSRPTPPIVPKPPAWQTGLAHLMHLALYLMMIGLPLAGWLILSAADKPVP
FFGLELPHLIGPNPDQAKFIKGWHERIGSWGYWLIGLHALAGLYHHYVQRDNTLLRMLPY
K