Protein Info for PP_1929 in Pseudomonas putida KT2440

Annotation: arsenite/antimonite transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details transmembrane" amino acids 25 to 43 (19 residues), see Phobius details amino acids 50 to 70 (21 residues), see Phobius details amino acids 94 to 131 (38 residues), see Phobius details amino acids 134 to 156 (23 residues), see Phobius details amino acids 176 to 198 (23 residues), see Phobius details amino acids 222 to 241 (20 residues), see Phobius details amino acids 247 to 266 (20 residues), see Phobius details amino acids 278 to 296 (19 residues), see Phobius details amino acids 308 to 330 (23 residues), see Phobius details amino acids 401 to 424 (24 residues), see Phobius details TIGR00935: arsenite/antimonite efflux pump membrane protein" amino acids 3 to 426 (424 residues), 693.3 bits, see alignment E=7.4e-213 PF02040: ArsB" amino acids 4 to 424 (421 residues), 511.3 bits, see alignment E=3e-157 PF03600: CitMHS" amino acids 12 to 368 (357 residues), 212.2 bits, see alignment E=1.1e-66

Best Hits

Swiss-Prot: 76% identical to ARSB2_ECOLX: Arsenical pump membrane protein (arsB) from Escherichia coli

KEGG orthology group: K03893, arsenical pump membrane protein (inferred from 100% identity to ppu:PP_1929)

MetaCyc: 75% identical to arsenite/antimonite:H+ antiporter (Escherichia coli K-12 substr. MG1655)
RXN-22385; RXN3O-9786

Predicted SEED Role

"Arsenic efflux pump protein" in subsystem Arsenic resistance

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LK2 at UniProt or InterPro

Protein Sequence (427 amino acids)

>PP_1929 arsenite/antimonite transporter (Pseudomonas putida KT2440)
MLIAFLIFLVTIVLVIWQPKGLGVGWSAALGAVVALLAGVVSLSDIPVVWQIVWNATATF
IAVIIISLLLDEAGFFEWAALHVARWGKGSSRKLFAFIILLGAAVSALFANDGAALILTP
IVIAMLLALRFSPAATLAFVMAAGFIADTASLPLVVSNLVNIVSADYFGIGFSEYASVMV
PVNLVSIAATLGMLLWFFRKDLPRTYELDQLKAPEAAIRDKATFVAGWWVLALLLVGFFA
IEPLGVPISAIAAACALILYLIAARGHVISTRKVLKDAPWQVVVFSLGMYLVVYGLRNAG
LTEYLTQILNVFAGYGIWGASLGTGLLAAGLSSAMNNMPTVLVGALSIQSAEATGIVREA
MVYANVIGCDLGPKITPIGSLATLLWLHVLARKNIVITWGYYFRTGIVLTLPILLATLAA
LAIRLSF