Protein Info for PP_1908 in Pseudomonas putida KT2440

Annotation: putative Peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 transmembrane" amino acids 42 to 63 (22 residues), see Phobius details amino acids 144 to 162 (19 residues), see Phobius details amino acids 174 to 191 (18 residues), see Phobius details PF00574: CLP_protease" amino acids 88 to 142 (55 residues), 20.9 bits, see alignment E=2.9e-08 PF01343: Peptidase_S49" amino acids 144 to 287 (144 residues), 106.9 bits, see alignment E=1e-34

Best Hits

KEGG orthology group: K04773, protease IV [EC: 3.4.21.-] (inferred from 100% identity to ppu:PP_1908)

Predicted SEED Role

"Periplasmic serine proteases (ClpP class)"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LM2 at UniProt or InterPro

Protein Sequence (329 amino acids)

>PP_1908 putative Peptidase (Pseudomonas putida KT2440)
MADEWKAPEAEPGEREERNSWKLLEKTLLASVQEQRRSRRWGIFFKLLTFVYLFGILALF
SPLMDMDKAASRSASHTALVEVRGVIADQEAASADNIVKSLREAFKDDKTKAVVMRINSP
GGSPVQAGYVYDEIRRLRAEYPAIKLYAVIADLGASGAYYIASAADEIYADKASLVGSIG
VTAAGYGFVGTMEKLGVERRAYTSGEHKAFLDPFSPEKPEETRFWQGVLDTTHRQFIAMV
KQGRGERLKDKEHPELFSGLIWSGEQAKALGLVDGLGSASYVAREIVGEKELVDFTVQES
PFDRFSKRVGASVAEHLAMWMGFQGPQLR