Protein Info for PP_1900 in Pseudomonas putida KT2440

Annotation: Tetraacyldisaccharide 4'-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02606: LpxK
  • amino acids 20 to 327 (308 residues), 374.2 bits, E=2.7e-116
• TIGR00682: tetraacyldisaccharide 4'-kinase
  • amino acids 28 to 316 (289 residues), 261.9 bits, E=3.7e-82

Best Hits

Swiss-Prot: 100% identical to LPXK_PSEPK: Tetraacyldisaccharide 4'-kinase (lpxK) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00912, tetraacyldisaccharide 4'-kinase [EC: 2.7.1.130] (inferred from 100% identity to ppu:PP_1900)

Predicted SEED Role

"Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130)" in subsystem KDO2-Lipid A biosynthesis or Lipopolysaccharide-related cluster in Alphaproteobacteria (EC 2.7.1.130)

MetaCyc Pathways

• lipid IV_A biosynthesis (P. gingivalis) (9/9 steps found)
• superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
• lipid IV_A biosynthesis (E. coli) (6/6 steps found)
• lipid IV_A biosynthesis (H. pylori) (6/6 steps found)
• lipid IV_A biosynthesis (P. putida) (6/6 steps found)
• lipid IV_A biosynthesis (Vibrio cholerae serogroup O1 El Tor) (6/6 steps found)
• lipid IV_A biosynthesis (generic) (6/6 steps found)
• lipid IV_A biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing) (5/6 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (16/25 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.130

Sequence Analysis Tools

See Q88LM9 at UniProt or InterPro

Protein Sequence (334 amino acids)

>PP_1900 Tetraacyldisaccharide 4'-kinase (Pseudomonas putida KT2440)
MMAFADRLLAAWYAGHPALALLRPLEALYRRVVTRKRARFLRGDSASYRAPVPVIVVGNI
TVGGTGKTPMILWLIEHCRRQGLKVGVVSRGYGAKPPQLPWRVQADQAADQAGDEPLLIV
QRTGVPLMIDPDRSRAVQALLASELLDLILCDDGMQHYRLARDLELVLIDAARGLGNGRC
LPAGPLREPADRLREADAVLFNGASEDRTDGFGFCLQPSALVNVRTGERRALDHFPAGQR
LHAVAGIGNPQRFFNTLLGLNWQPVPHPFADHAQFSARSLAFSPPLPLVMTEKDAVKCRA
FAADDWWYLAVEAQPTPAFSAWFDNQLQRLLRKP