Protein Info for PP_1850 in Pseudomonas putida KT2440

Annotation: putative uncharacterized transporter YgaY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 39 to 64 (26 residues), see Phobius details amino acids 76 to 97 (22 residues), see Phobius details amino acids 100 to 101 (2 residues), see Phobius details amino acids 103 to 122 (20 residues), see Phobius details amino acids 134 to 155 (22 residues), see Phobius details amino acids 162 to 181 (20 residues), see Phobius details amino acids 216 to 236 (21 residues), see Phobius details amino acids 243 to 264 (22 residues), see Phobius details amino acids 274 to 292 (19 residues), see Phobius details amino acids 298 to 317 (20 residues), see Phobius details amino acids 337 to 357 (21 residues), see Phobius details amino acids 363 to 384 (22 residues), see Phobius details PF07690: MFS_1" amino acids 15 to 316 (302 residues), 112.8 bits, see alignment E=1.8e-36 PF00083: Sugar_tr" amino acids 37 to 177 (141 residues), 31 bits, see alignment E=1.3e-11

Best Hits

Swiss-Prot: 61% identical to YGAY_ECOLI: Putative uncharacterized transporter YgaY (ygaY) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1850)

Predicted SEED Role

"MFS permease protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88LS8 at UniProt or InterPro

Protein Sequence (395 amino acids)

>PP_1850 putative uncharacterized transporter YgaY (Pseudomonas putida KT2440)
MHTPTLSRALILLMATATGLAVASNYYAQPLLHSIAQQFGLSTASAGSIVIAAQLSYGAG
LLLLAPLGDLFEQRRLITMMTVVSTLGLVISACAPSLPWLLLGTALTGLFSVVAQILVPM
AATLSEPHQRGRAVGTLMSGLLLGILLARTAAGFMAELGGWRSIYVLAAALMALTALALY
RSLPQHHSHAGLKYPALIGSVFRLFIEEPVLRLRSLLGLLAFSLFALFWTPLAFLLANGP
YHYSDAVIGLFGLAGAAGALSANWAGRLADRGKGSLGTTVGLVVLLLSWVPLGFAEQSLL
ALLLGVLMLDLAVQLVHVSNQNAVIALRPEARTRLNAGYITCYFIGGALGSLLGTQLFQR
QGWMGIVVAGLVIGGLGLLVWGLAERKRKNTLQVA