Protein Info for PP_1784 in Pseudomonas putida KT2440
Annotation: dTDP-4-dehydrorhamnose reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 55% identical to RMLD_SALTY: dTDP-4-dehydrorhamnose reductase (rfbD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 100% identity to ppu:PP_1784)Predicted SEED Role
"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)
MetaCyc Pathways
- O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
- dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
- superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (5/19 steps found)
- superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.133
Use Curated BLAST to search for 1.1.1.133
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88LZ2 at UniProt or InterPro
Protein Sequence (300 amino acids)
>PP_1784 dTDP-4-dehydrorhamnose reductase (Pseudomonas putida KT2440) MKILLLGKNGQVGWELQRALAPLGEVIALDRQGAEGLCGDLSNLDGLAATIRQLAPDVIV NAAAYTAVDKAESDQALAAMINAAAPAVLARETAALGAWLIHYSTDYVFDGSGSQRWEET APTGPLSVYGRTKLEGEHAILASGAKAVVLRTSWVYAARGHNFAKTMLRLAAERETLSVV ADQFGAPTGADLIADVTAHILRQIFNGQDNRHLAGIYHLAASGETSWHGFAQFVLAHAQR TGVALKVTADKVAAISTEAYPVPAPRPRNSRLALGKLENTFNFKMPLWEQGVQRMLDEIQ