Protein Info for PP_1767 in Pseudomonas putida KT2440

Annotation: DNA gyrase, subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 923 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 639 (632 residues), 982.8 bits, see alignment E=5.8e-300 PF00521: DNA_topoisoIV" amino acids 32 to 508 (477 residues), 545.9 bits, see alignment E=7e-168 PF03989: DNA_gyraseA_C" amino acids 540 to 586 (47 residues), 36.4 bits, see alignment 2.9e-13 amino acids 591 to 638 (48 residues), 35.3 bits, see alignment 6.4e-13 amino acids 689 to 732 (44 residues), 35.2 bits, see alignment 6.5e-13 amino acids 737 to 781 (45 residues), 52.9 bits, see alignment 2e-18 amino acids 787 to 833 (47 residues), 42.8 bits, see alignment 2.8e-15 amino acids 837 to 883 (47 residues), 43.6 bits, see alignment 1.6e-15

Best Hits

Swiss-Prot: 85% identical to GYRA_PSEAE: DNA gyrase subunit A (gyrA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 67% identity to abo:ABO_1751)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88M08 at UniProt or InterPro

Protein Sequence (923 amino acids)

>PP_1767 DNA gyrase, subunit A (Pseudomonas putida KT2440)
MGELAKEILPVNIEDELRQSYLDYAMSVIVGRALPDARDGLKPVHRRVLYAMSELGNDWN
KPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAM
RYTEVRMTKLAHELLADLHKETVDWVPNYDGTEQIPAVMPTRIPNLLVNGSSGIAVGMAT
NIPPHNLGEVIDGCLALIDNPEVTIDELMQHIPGPDFPTAGIINGRQGIIEAYRTGRGRI
YMRARSEIEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKIEGITELRDESDKD
GMRIVIELRRGEVPEVVLNNLYQQTQLQSVFGINVVALVDGRPRLLNLKDLLEAFVRHRR
EVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKASPTPSEAKEALVSTPWESSA
VQVMVERAGADSCRPEDLPEQYGLHDGKYFLSPEQAQAILDLRLHRLTGLEHEKLLAEYQ
EILEQIGELIRILSSAERLMEVIREELEAIRAEYGDVRRTEILNASHDLNYGDMIPEEER
VVTISHGGYAKTQPLSAYQAQRRGGKGKSATGVKDEDYIEHLLVANSHATLLLFSSKGKV
YWKKTYEIPEASRAARGRPLVNLLPLEEGERITAMLQIDLEALQQNADLDEELDEAEDTV
LEGELVEAEEVDEEDGDTPEWVAEPTGAYIFMATASGTVKKTPLVQFARPRSNGLIALKL
KEGDTLIAAAITDGAKEVMMFSDAGKVIRFAESVVREMGRNARGVRGMKLGKGQRIISML
IPESGSQILTASERGFGKRTPLSKFPRRGRGGQGVIAMGTKGRNGLLIGAIQVQEGEEIM
LISDQGTLVRTRVGEVSSLSRNTQGVTLIKLAADETLVGLERIQEPSEDELDEVIETDEE
GVEAVAPDNENEEAAGADEAPQE