Protein Info for PP_1678 in Pseudomonas putida KT2440

Annotation: bifunctional cobinamide-P guanylyltransferase - adenosylcobinamide kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02283: CobU
  • amino acids 4 to 166 (163 residues), 205.6 bits, E=2e-65

Best Hits

Swiss-Prot: 73% identical to COBP_PSEAE: Bifunctional adenosylcobalamin biosynthesis protein CobP (cobP) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02231, adenosylcobinamide kinase / adenosylcobinamide-phosphate guanylyltransferase [EC: 2.7.1.156 2.7.7.62] (inferred from 99% identity to ppg:PputGB1_1277)

MetaCyc: 48% identical to CobP (Pseudomonas denitrificans (nom. rej.))
Adenosylcobinamide-phosphate guanylyltransferase. [EC: 2.7.7.62]; Adenosylcobinamide kinase. [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]; 2.7.1.156 [EC: 2.7.7.62, 2.7.1.156]

Predicted SEED Role

"Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.7.7.62)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
• adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
• superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
• adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
• adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
• adenosylcobinamide-GDP salvage from assorted adenosylcobamides (1/2 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.156 or 2.7.7.62

Sequence Analysis Tools

See Q88M96 at UniProt or InterPro

Protein Sequence (173 amino acids)

>PP_1678 bifunctional cobinamide-P guanylyltransferase - adenosylcobinamide kinase (Pseudomonas putida KT2440)
MRNLILGGARSGKSRLAEQLASASGLPVTYIATSQPLDGEMNERVLLHRQRRPDDWGLIE
EPLALAAVLRAEAAEGRCLLVDCLTLWLTNLLMLEDDQRLAEERDALLACLEQLPGTLIL
VSNETGLGVVPMGELTRRYVDLAGWLHQAVAERCQRVVLTVAGLPLILKGPAL