Protein Info for PP_1668 in Pseudomonas putida KT2440

Annotation: regulator of DnaA that prevents premature reinitiation of DNA replication

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 235 TIGR03420: DnaA regulatory inactivator Hda" amino acids 6 to 233 (228 residues), 285.3 bits, see alignment E=1.6e-89 PF00308: Bac_DnaA" amino acids 16 to 213 (198 residues), 67.5 bits, see alignment E=8.1e-23

Best Hits

Swiss-Prot: 47% identical to Y122B_HAEIN: Uncharacterized protein HI_1225.1 (HI_1225.1) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K10763, DnaA-homolog protein (inferred from 99% identity to ppg:PputGB1_1266)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MA6 at UniProt or InterPro

Protein Sequence (235 amino acids)

>PP_1668 regulator of DnaA that prevents premature reinitiation of DNA replication (Pseudomonas putida KT2440)
MKPPIQLPLGVRLRDDATFINYYPGANAAALGYVERLCEADAGWTESLIYLWGKQGVGRS
HLLQAATHRFQQRGEPAVYLPLAQLLDRGVELLDYLAQYELVCIDDLHVIAGKADWEEAM
FHLFNRLRDSGRRLLLAASASPRELPIKLPDLKSRLTLALVFQMRGLSDEDKLRALQLRA
SRRGLHLTDEVGHFILTRGARSMSALFDLLERLDQASLQAQRKLTIPFLKETLGW