Protein Info for PP_1661 in Pseudomonas putida KT2440

Annotation: putative Dehydrogenase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 PF00890: FAD_binding_2" amino acids 13 to 336 (324 residues), 20.7 bits, see alignment E=5.3e-08 PF13450: NAD_binding_8" amino acids 16 to 44 (29 residues), 24.9 bits, see alignment (E = 4.9e-09) PF00732: GMC_oxred_N" amino acids 240 to 368 (129 residues), 39.1 bits, see alignment E=1.8e-13 PF05199: GMC_oxred_C" amino acids 482 to 613 (132 residues), 65.4 bits, see alignment E=2.1e-21

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1661)

Predicted SEED Role

"Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site" in subsystem Respiratory dehydrogenases 1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MB3 at UniProt or InterPro

Protein Sequence (752 amino acids)

>PP_1661 putative Dehydrogenase subunit (Pseudomonas putida KT2440)
MSNVQTQNIETADVIVVGAGMAGSVMAYQLGLAGLKVLVLESGPAIPPNRSEYLERFYTA
VLKAPESPYPPEQSEQTPATQFAPRATIQDLITSGSQDPNKAQKSYLVQKGPHLFASTYE
RVGGGTMWHWMGTALRLLPNDFRMRTAYNVGVDWPISYDDLQTAYCRAEEEMGVSADVAS
QTYLGVTFPQGYKYSMHGIPPSLVDQGLGKHVTGTVYQSPEKGPDGKPYPGVTLNVRQTP
AGRNSQNDNGRRVCAGNTSCTPICPIQAKYDATVTMAKALDTGNVRILYQTVASQVQVDA
NGVTGIDFIQYQNNGTRQNGTAQGKRYVIAAHAIETPKLLLNSIGPNSPKGVANSSGQVG
QALADHPVYLAWGLMPEGKPLFPFRGPLSTSGIEDMRDGPFRSQRAAWRIEIGNEGWNWS
VNDPYTSVCDFIDGTNHGGANPSNLALSGQALVQQLNSVLTRQFRLGFLVEQVEKDPDNA
LCRVERSTEFSDGLGLPRPQITYELSDYTKEGFKQARLAATYIITELMGATELTDLNPKL
EPGSQTVFEYDGQNYAFYGAGHLMGTYRMGSDRTKSVVDKDQRSWDHPNLFLVGDGVFPT
TGTANPTLTITALTFQAADVVASDLLKRTVQVQANQAWQGTGMQVNGQSWQLAVCTGGQW
TANPATGMVGAAGNPRLIAKPGYALPGQPEGALIGRIGNSGVPFLVGNQVQLPRGQQGEL
QLCINDDLDGRYGAGLSDNLGSLSVEVRFGAV