Protein Info for PP_1593 in Pseudomonas putida KT2440

Annotation: Uridylate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02075: UMP kinase
  • amino acids 12 to 243 (232 residues), 329.1 bits, E=7.5e-103
• PF00696: AA_kinase
  • amino acids 13 to 222 (210 residues), 107.9 bits, E=3.5e-35

Best Hits

Swiss-Prot: 100% identical to PYRH_PSEPK: Uridylate kinase (pyrH) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K09903, uridylate kinase [EC: 2.7.4.22] (inferred from 99% identity to pen:PSEEN4218)

MetaCyc: 70% identical to UMP kinase (Escherichia coli K-12 substr. MG1655)
Cytidylate kinase. [EC: 2.7.4.14, 2.7.4.22]

Predicted SEED Role

"Uridine monophosphate kinase (EC 2.7.4.22)" (EC 2.7.4.22)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• superpathway of pyrimidine nucleobases salvage (4/4 steps found)
• UTP and CTP de novo biosynthesis (3/3 steps found)
• CMP phosphorylation (2/2 steps found)
• superpathway of pyrimidine ribonucleosides salvage (7/10 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.14

Use Curated BLAST to search for 2.7.4.14 or 2.7.4.22

Sequence Analysis Tools

See Q88MH8 at UniProt or InterPro

Protein Sequence (247 amino acids)

>PP_1593 Uridylate kinase (Pseudomonas putida KT2440)
MAQQVSGRQPRYKRILLKLSGEALMGSEDFGIDPKVLDRMALEVGQLVGIGVQVGLVIGG
GNLFRGAALSAAGMDRVTGDHMGMLATVMNGLAMRDALERSNIPALVMSAISMVGVTDHY
DRRKAIRHLNSGDVVIFSAGTGNPFFTTDSAACLRAIEIDADVVLKATKVDGVYTADPFK
DPHAEKFDHLTYDEVLDRKLGVMDLTAICLCRDHKMPLRVFNMNKPGALLNIVVGGAEGT
LIEEGQA