Protein Info for PP_1525 in Pseudomonas putida KT2440

Annotation: Succinyl-diaminopimelate desuccinylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 TIGR01246: succinyl-diaminopimelate desuccinylase" amino acids 11 to 381 (371 residues), 531.8 bits, see alignment E=4.3e-164 PF01546: Peptidase_M20" amino acids 69 to 378 (310 residues), 139.4 bits, see alignment E=1.6e-44 PF07687: M20_dimer" amino acids 183 to 289 (107 residues), 103.4 bits, see alignment E=6.2e-34

Best Hits

Swiss-Prot: 100% identical to DAPE_PSEPK: Succinyl-diaminopimelate desuccinylase (dapE) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01439, succinyl-diaminopimelate desuccinylase [EC: 3.5.1.18] (inferred from 99% identity to ppg:PputGB1_1130)

MetaCyc: 60% identical to succinyl-diaminopimelate desuccinylase (Escherichia coli K-12 substr. MG1655)
Succinyl-diaminopimelate desuccinylase. [EC: 3.5.1.18]

Predicted SEED Role

"N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 3.5.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.18

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MP5 at UniProt or InterPro

Protein Sequence (383 amino acids)

>PP_1525 Succinyl-diaminopimelate desuccinylase (Pseudomonas putida KT2440)
MTAPAELSPTLQLACDLIRRPSVTPVDADCQAQMMNRLGAVGFQLEPMRIEDVDNFWATH
GSQDGPVLCFAGHTDVVPTGPVQQWQHEPFEALIDADGMLCGRGAADMKGSLASMVIASE
RFVQDYPNHRGKVAFLITSDEEGPAHHGTKAVVERLKARNERLDWCIVGEPSSTTLLGDV
VKNGRRGSLGAKLTIRGKQGHVAYPHLARNPIHLAAPALAELAAEHWDEGNAFFPPTSFQ
ISNLNSGTGATNVVPGELTALFNFRFSTESTVEGLQARVSAILDKHELDWSVDWALSGLP
FLTEPGELLDAVAASIKGVTGRDTQPSTSGGTSDGRFIATMGTQVVELGPVNATIHQVDE
RILASDLDLLTEIYYQTLVRLLA