Protein Info for PP_1496 in Pseudomonas putida KT2440

Annotation: Lysine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 TIGR00499: lysine--tRNA ligase" amino acids 18 to 499 (482 residues), 665.6 bits, see alignment E=2.2e-204 PF01336: tRNA_anti-codon" amino acids 68 to 141 (74 residues), 41.6 bits, see alignment E=1.5e-14 PF00152: tRNA-synt_2" amino acids 158 to 496 (339 residues), 315.8 bits, see alignment E=4.9e-98

Best Hits

Swiss-Prot: 100% identical to SYK_PSEPK: Lysine--tRNA ligase (lysS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 100% identity to ppg:PputGB1_1101)

MetaCyc: 62% identical to lysine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MS3 at UniProt or InterPro

Protein Sequence (500 amino acids)

>PP_1496 Lysine--tRNA ligase (Pseudomonas putida KT2440)
MSDLKTESQDLQQEENALIALRKEKLAAERAKGNAFPNDFRRDSYCNDLQKQYADKTKEE
LEAAAIPVKVAGRIMLNRGSFMVIQDMTGRIQVYVNRKTLPEETLAAVKTWDLGDIISAE
GTLARSGKGDLYVEMTNVRLLTKSLRPLPDKHHGLTDTEQRYRQRYVDLMVNEETRHTFR
VRSQVISHIRKFLIERDFLEVETPMLQTIPGGAAAKPFETHHNALDMAMFLRIAPELYLK
RLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYRDNMDLTEELFRELAQLV
LGSTDVPYGDKVFHFGEPFVRLSVFDSILKYNPELTAADLQDVDRAREIARKAGAKVLGH
EGLGKLQVMIFEELVEHKLEQPHFITEYPFEVSPLARRNDDNPAVTDRFELFIGGREIAN
AYSELNDAEDQAERFLAQVAEKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVML
LTNSPSIRDVILFPHMRPQA