Protein Info for PP_1494 in Pseudomonas putida KT2440

Annotation: Response regulator/GGDEF domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 PF00072: Response_reg" amino acids 20 to 133 (114 residues), 68.7 bits, see alignment E=4.8e-23 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 166 to 332 (167 residues), 164.4 bits, see alignment E=9.6e-53 PF00990: GGDEF" amino acids 171 to 331 (161 residues), 165.5 bits, see alignment E=8.7e-53

Best Hits

KEGG orthology group: K11444, two-component system, chemotaxis family, response regulator WspR [EC: 2.7.7.65] (inferred from 100% identity to ppu:PP_1494)

Predicted SEED Role

"diguanylate cyclase (GGDEF domain) with PAS/PAC sensor"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MS4 at UniProt or InterPro

Protein Sequence (335 amino acids)

>PP_1494 Response regulator/GGDEF domain protein (Pseudomonas putida KT2440)
MMNDLPIEGFATTNENSAMVLLVDDQAMIGEAVRRGLANEDNIDFHFCADPHQAVAQAMR
IKPTVILQDLIMPGLDGLTLVREYRNNPVTQDIPIIVLSTKEDPLVKSAAFAAGANDYLV
KLPDTIELVARIRYHSRSYLTLLQRDEAYRALRVSQQQLLDSNLMLQRLMNSDGLTGLSN
RRHFDEYLELEWRRAMREQQQLSLLMIDVDYFKVYNDSFGHLAGDEALRQVAEAIRSSCS
RPTDLPARYGGEEFALVLPNTSPGGARLVAEKLRQTVLGLGIPHTAPTADTFLTVSIGLA
TQTPAIGSHCRQLISAADKGLYLAKNGGRNQVGIA