Protein Info for PP_1458 in Pseudomonas putida KT2440

Annotation: putative inner membrane metabolite transport protein YhjE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 transmembrane" amino acids 35 to 52 (18 residues), see Phobius details amino acids 59 to 81 (23 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 119 to 143 (25 residues), see Phobius details amino acids 162 to 185 (24 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details amino acids 243 to 263 (21 residues), see Phobius details amino acids 283 to 304 (22 residues), see Phobius details amino acids 312 to 332 (21 residues), see Phobius details amino acids 338 to 362 (25 residues), see Phobius details amino acids 374 to 394 (21 residues), see Phobius details amino acids 405 to 424 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 22 to 232 (211 residues), 90.9 bits, see alignment E=8.9e-30 amino acids 257 to 432 (176 residues), 49.2 bits, see alignment E=3.9e-17 PF07690: MFS_1" amino acids 25 to 370 (346 residues), 82.7 bits, see alignment E=2.4e-27 amino acids 284 to 424 (141 residues), 46.3 bits, see alignment E=2.8e-16 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 27 to 418 (392 residues), 446.2 bits, see alignment E=5.7e-138

Best Hits

Swiss-Prot: 55% identical to YHJE_ECOLI: Inner membrane metabolite transport protein YhjE (yhjE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1458)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MW0 at UniProt or InterPro

Protein Sequence (432 amino acids)

>PP_1458 putative inner membrane metabolite transport protein YhjE (Pseudomonas putida KT2440)
MTSSTYAQAPAAPVNSPARVATASFIGTAIEFYDFYVYATAAALVIGPVFFPSGSGTAQM
LAAFLTFGIAFLARPLGSALFGHFGDRIGRKSTLVASLLLMGVSTTAIGVLPGYDSIGVW
APIILCLLRFGQGLGLGGEWGGAALLATENAPEGKRAWFGMFPQLGPSIGFLAANGLFLT
LALVLSDEQFREWGWRIPFLLSAALVLVGLYVRLKLEESPVFAKAVARHERVKMPVVDLF
SKYWLPTLLGAAAMVVCYALFYISTVFSLSYGVTTLGYSRETFLGLLCFAVVFMALATPL
SAWLSDRYGRKPVLIVGGLLAIASGFTMEPLLTSGSTAGVALFLAIELFLMGVTFAPMGA
LLPELFPTHVRYTGASAAYNLGGIVGASAAPFFAQKLVSMGGLSWVGGYVSVAAVISLIA
VLCLKETRNTTL