Protein Info for PP_1445 in Pseudomonas putida KT2440

Annotation: carbohydrate-selective porin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF04966: OprB" amino acids 72 to 444 (373 residues), 447.5 bits, see alignment E=2.6e-138

Best Hits

Swiss-Prot: 68% identical to PORB_PSEAE: Porin B (oprB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K07267, porin (inferred from 100% identity to ppu:PP_1445)

Predicted SEED Role

"Glucose-selective porin OprB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MX3 at UniProt or InterPro

Protein Sequence (444 amino acids)

>PP_1445 carbohydrate-selective porin (Pseudomonas putida KT2440)
MFQLPKTCYIGLALSALATPAGASEMFASDSPWMLGDWGGTRSELLEKGYDFTLGYTGEM
GSNLHGGYDHDRTARYSDQFTFGSHLDLEKILGWHDTEFQLTVTERHGDNISNDRINDPR
VGGFTSAQEVWGRGETWRLTQMWIKQKYFDGALDVKFGRFGEGEDFNSFPCDFQNLAFCG
SQVGNWVGGIWYNWPVSQWALRVRYNLTPELYAQVGVFEQNPSNLESGNGFKLSGSGTQG
AVMPFELVWTPRIQGLKGEYRAGYYYSNAKAQDVLKDSNGQPAALSGAAYRSSSSKHGLW
IGAQQQVTSLASDQSRGLSVFANATVHDKKTNAIDNYVQAGLVFKGPFDARAKDDIGFAL
ARVHVNPAYRKNARLVNQAAGLYDYDNPGFLPVQDTEYSAELYYGIHLADWLTVRPNLQY
IRHPGGVSQVDGALIGGLKIQSSF