Protein Info for PP_1437 in Pseudomonas putida KT2440

Annotation: Heavy metal sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 161 to 185 (25 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 4 to 458 (455 residues), 458.5 bits, see alignment E=1.4e-141 PF00672: HAMP" amino acids 183 to 235 (53 residues), 40 bits, see alignment 6.1e-14 PF00512: HisKA" amino acids 241 to 303 (63 residues), 54 bits, see alignment E=2.1e-18 PF02518: HATPase_c" amino acids 349 to 459 (111 residues), 101.3 bits, see alignment E=6.8e-33

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to ppf:Pput_4284)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MY1 at UniProt or InterPro

Protein Sequence (460 amino acids)

>PP_1437 Heavy metal sensor histidine kinase (Pseudomonas putida KT2440)
MKNASLSLRLGLSVTLMGAALVLLLACLAVFALDHELDSRARKDLARKMLQVEHNLRVDL
RSEDLGSRAHPLLDLVMGHDNLSLSVLALDGRHPHLLSLGPALESRVGELHTDARLAFHA
WRDSGGNQILTVTRLMRLRDNTPVKVMMSLNRADDNALLQAYLHSTLLALPLLLVLIGAG
AWWLMQRGLKPLRHFRRIAGQVSAQDLQHRLPATSLPQELADLAGSINVMLDRLDQGVRQ
LSQFSDDLAHELRTPLSNLMGKAQVTLARERDNANYREVLEDSIEELTRLNRIINDMLFL
AQVSQPHTQVALKPLALSDEAARVVELFAYSAELKEIELRVQGWGTALGDRLMFQRALSN
LLSNAIRHSPEGKPVALRIERSGSEVILSVENQGPGVPAEHQSRLFERFYRVGSGRSRLE
GGTGLGLAIVKSIMQLHGGRVEVSSCPLGPTRFTLVFPAE