Protein Info for PP_1434 in Pseudomonas putida KT2440

Annotation: GTPase Era

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 11 to 168 (158 residues), 100 bits, E=1.2e-32
• TIGR00436: GTP-binding protein Era
  • amino acids 12 to 282 (271 residues), 328.8 bits, E=2.4e-102
• PF00009: GTP_EFTU
  • amino acids 14 to 177 (164 residues), 32.2 bits, E=3.3e-11
• PF01926: MMR_HSR1
  • amino acids 14 to 128 (115 residues), 90.9 bits, E=2.5e-29
• PF02421: FeoB_N
  • amino acids 14 to 172 (159 residues), 63 bits, E=9.5e-21
• PF04548: AIG1
  • amino acids 15 to 122 (108 residues), 36.6 bits, E=1.3e-12
• PF07650: KH_2
  • amino acids 212 to 287 (76 residues), 74.8 bits, E=1.6e-24

Best Hits

Swiss-Prot: 100% identical to ERA_PSEPK: GTPase Era (era) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03595, GTP-binding protein Era (inferred from 100% identity to ppu:PP_1434)

MetaCyc: 60% identical to 30S ribosomal subunit maturation GTPase Era (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Era" in subsystem Bacterial Cell Division or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See Q88MY4 at UniProt or InterPro

Protein Sequence (302 amino acids)

>PP_1434 GTPase Era (Pseudomonas putida KT2440)
MAMTDSNPTRCGYVAIVGRPNVGKSTLLNHILGQKLAITSRKPQTTRHNMLGIKTEGDVQ
AIYVDTPGMHKANDKALNRYMNRNASAALKDVDVVIFVVDRTKWTDEDQLVLERVQYVTG
PLIIAVNKTDRMEEKAELIPHLQWLQEQLPNAEVMPISAQQGHNLDALEAQIAKHLPEND
HFFPEDQITDRSSRFLAAELVREKIMRQLGAELPYQITVEIEEFKQQGHVLHIHALILVE
RDGQKKIIIGDKGERIKRIGSEARKDMEVLFDSKVMLNLWVKVKGGWSDDERALRSLGYG
DL