Protein Info for PP_1423 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 TIGR03317: folate-binding protein YgfZ" amino acids 170 to 235 (66 residues), 85.3 bits, see alignment E=1.1e-28

Best Hits

KEGG orthology group: K06980, (no description) (inferred from 100% identity to ppu:PP_1423)

Predicted SEED Role

"Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88MZ5 at UniProt or InterPro

Protein Sequence (313 amino acids)

>PP_1423 conserved protein of unknown function (Pseudomonas putida KT2440)
MADSAFFCPLSHEGILAVRGSDAGKFLQGQLTCNINYLSQEHASLGARCMVKGRMQSSFR
IVPEGNGYLLAMASELLDAQLADLKKYAVFSKATLTDESTAWARFGLQGGDAALQALGLV
VPAAAGSTVRHDGLIAIAVSAGRVELWVPVADAEPVRQALAAALPEGTLNDWLLGQIRAG
IGQVMGPTRELFIPQMINLQAVDGVSFKKGCYTGQEIVARMQYLGKLKRRQYRLALDQQA
IPAPGAEIFSPTHGSSVGEVVIAAGNGTGCELLAVLSAEAVEDGHLHLGSLEGPRLQVLT
LPYELDRDREIQR