Protein Info for PP_1385 in Pseudomonas putida KT2440
Annotation: Probable efflux pump membrane transporter TtgB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to MEPB_PSEPU: Multidrug/solvent efflux pump membrane transporter MepB (mepB) from Pseudomonas putida
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 64% identity to abo:ABO_0964)MetaCyc: 60% identical to multidrug efflux pump RND permease MdtF (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-359; TRANS-RXN-367
Predicted SEED Role
"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q88N31 at UniProt or InterPro
Protein Sequence (1050 amino acids)
>PP_1385 Probable efflux pump membrane transporter TtgB (Pseudomonas putida KT2440) MSKFFIDRPIFAWVIALVIMLVGALSILKLPINQYPSIAPPAIAIAVTYPGASAQTVQDT VVQVIEQQLNGIDNLRYVSSESNSDGSMTITATFEQGTNPDTAQVQVQNKLNLATPLLPQ EVQQQGIRVTKAVKNFLLVIGLVSEDGSMTKDDLANYIVSNMQDPISRTAGVGDFQVFGA QYAMRIWLDPAKLNKFQLTPVDVKTAVAAQNVQVSSGQLGGLPALPGTQLNATIIGKTRL QTAEQFESILLKVNKDGSQVRLGDVAQVGLGGENYAVSAQFNGKPASGLAVKLATGANAL DTAKALRETIKGLEPFFPPGVKAVFPYDTTPVVTESISGVIHTLIEAVVLVFLVMYLFLQ NFRATIITTMTVPVVLLGTFGILAAAGFSINTLTMFAMVLAIGLLVDDAIVVVENVERVM SEEGLPPKEATKRSMEQIQGALVGIALVLSAVLLPMAFFGGSTGVIYRQFSITIVSAMGL SVLVALIFTPALCATMLKPLKKGEHHTAKGGFFGWFNRNFDRSVNGYERSVGAILRNKVP FLLAYALIVVGMIWLFARIPTAFLPEEDQGVLFAQVQTPAGSSAERTQVVVDQMREYLLK DEADTVSSVFTVNGFNFAGRGQSSGMAFIMLKPWDERSKENSVFALAQRAQQHFFTFRDA MVFAFAPPAVLELGNATGFDVFLQDRGGVGHEKLMEARNQFLAKAAQSKILSAVRPNGLN DEPQYQLTIDDERASALGVTIADINNTLSIALGASYVNDFIDRGRVKKVYIQGEPSARMS PEDLQKWYVRNGAGEMVPFSSFAKGEWTYGSPKLSRYNGVEAMEILGAPAPGYSTGEAMA EVERIAGELPSGIGFSWTGMSYEEKLSGSQMPALFALSVLFVFLCLAALYESWSIPIAVV LVVPLGIIGALIATSLRGLSNDVYFLVGLLTTIGLAAKNAILIVEFAKELHEQGRSLYDA AIEACRMRLRPIIMTSLAFILGVVPLTIASGAGAGSQHAIGTGVIGGMISATVLAIFWVP LFFVAVSSLFGSKEPEKDVTPENPRYEAGQ