Protein Info for PP_1380 in Pseudomonas putida KT2440

Annotation: 3-oxoadipate enol-lactonase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02427: 3-oxoadipate enol-lactonase
  • amino acids 11 to 260 (250 residues), 330 bits, E=4.5e-103
• PF00561: Abhydrolase_1
  • amino acids 22 to 246 (225 residues), 83.3 bits, E=3.4e-27
• PF12697: Abhydrolase_6
  • amino acids 31 to 243 (213 residues), 51.9 bits, E=2.5e-17
• PF12146: Hydrolase_4
  • amino acids 45 to 229 (185 residues), 42.6 bits, E=7.1e-15

Best Hits

Swiss-Prot: 47% identical to ELH1_ACIAD: 3-oxoadipate enol-lactonase 1 (pcaD) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01055, 3-oxoadipate enol-lactonase [EC: 3.1.1.24] (inferred from 99% identity to ppf:Pput_4343)

MetaCyc: 100% identical to subunit of 3-oxoadipate enol-lactone hydrolase (Pseudomonas putida)
3-oxoadipate enol-lactonase. [EC: 3.1.1.24]

Predicted SEED Role

"Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24)" in subsystem Catechol branch of beta-ketoadipate pathway or Chloroaromatic degradation pathway or Protocatechuate branch of beta-ketoadipate pathway (EC 3.1.1.24)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (9/9 steps found)
• superpathway of salicylate degradation (7/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• catechol degradation to β-ketoadipate (4/4 steps found)
• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• 4-methylcatechol degradation (ortho cleavage) (5/7 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• mandelate degradation to acetyl-CoA (11/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (19/35 steps found)
• superpathway of aerobic toluene degradation (13/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.24

Use Curated BLAST to search for 3.1.1.24

Sequence Analysis Tools

See Q88N36 at UniProt or InterPro

Protein Sequence (263 amino acids)

>PP_1380 3-oxoadipate enol-lactonase 2 (Pseudomonas putida KT2440)
MAHLQLADGVLNYQIDGPDDAPVLVLSNSLGTDLGMWDTQIPLWSQHFRVLRYDTRGHGA
SLVTEGPYSIEQLGRDVLALLDGLDIQKAHFVGLSMGGLIGQWLGIHAGERLHSLTLCNT
AAKIANDEVWNTRIDTVLKGGQQAMVDLRDASIARWFTPGFAQAQAEQAQRICQMLAQTS
PQGYAGNCAAVRDADYREQLGRIQVPALIVAGTQDVVTTPEHGRFMQAGIQGAEYVDFPA
AHLSNVEIGEAFSRRVLDFLLAH