Protein Info for PP_1378 in Pseudomonas putida KT2440

Annotation: alpha-ketoglutarate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 transmembrane" amino acids 26 to 47 (22 residues), see Phobius details amino acids 56 to 78 (23 residues), see Phobius details amino acids 90 to 110 (21 residues), see Phobius details amino acids 116 to 137 (22 residues), see Phobius details amino acids 155 to 177 (23 residues), see Phobius details amino acids 189 to 208 (20 residues), see Phobius details amino acids 237 to 257 (21 residues), see Phobius details amino acids 277 to 295 (19 residues), see Phobius details amino acids 307 to 325 (19 residues), see Phobius details amino acids 331 to 354 (24 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details amino acids 400 to 418 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 19 to 215 (197 residues), 94.7 bits, see alignment E=9.2e-31 amino acids 219 to 414 (196 residues), 43.2 bits, see alignment E=3.9e-15 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 24 to 413 (390 residues), 489.3 bits, see alignment E=4.5e-151 PF07690: MFS_1" amino acids 53 to 379 (327 residues), 98.5 bits, see alignment E=6e-32 PF12832: MFS_1_like" amino acids 257 to 413 (157 residues), 26.1 bits, see alignment E=6.8e-10

Best Hits

Swiss-Prot: 70% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K02625, MFS transporter, MHS family, dicarboxylic acid transporter PcaT (inferred from 100% identity to ppu:PP_1378)

MetaCyc: 70% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N38 at UniProt or InterPro

Protein Sequence (429 amino acids)

>PP_1378 alpha-ketoglutarate permease (Pseudomonas putida KT2440)
MTSTYYTGEERSKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPAFFPSDDPTVQLLNTA
GVFAAGFLMRPIGGWIFGRLADRHGRKNSLMISVLMMCFGSLMIACLPTYASIGTWAPAL
LLLARLIQGLSVGGEYGTTATYMSEVALRGQRGFFASFQYVTLIGGQLLAVLVVVILQQL
LTEDELRAWGWRIPFVVGAIAALISLMLRRSLHETSSAEARNDKDAGSIGGLFRNHAAAF
ITVLGYTAGGSLIFYTFTTYMQKYLVNTAGMTAKNASYVMTGALFLFMVVQPFFGMLSDR
IGRRNSMLLFGGLGTLCTVPLLMALKTVTSPIMAFVLISLALCIVSFYTSISGLVKAEMF
PPQVRALGVGLAYAVANAAFGGSAEYVALGLKTLGMENTFYWYVTAMMAIAFLFSLRLPK
QAAYLHHDD