Protein Info for PP_1346 in Pseudomonas putida KT2440
Annotation: Glutamate N-acetyltransferase/Amino-acid acetyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ARGJ_PSEPK: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 100% identity to ppf:Pput_4378)Predicted SEED Role
"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- superpathway of arginine and polyamine biosynthesis (14/17 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- L-ornithine biosynthesis I (5/5 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.1
Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P59612 at UniProt or InterPro
Protein Sequence (405 amino acids)
>PP_1346 Glutamate N-acetyltransferase/Amino-acid acetyltransferase (Pseudomonas putida KT2440) MAVGLGPLPTLHPVPGFELGIASAGIKRPGRKDVVVMRCAEGSSVAGVFTLNAFCAAPVI LSKQRVQGTVRYLLTNTGNANAGTGAPGLAAAERTCAKLAELAGVPAESVLPFSTGVIGE PLPVEKIEGALQAALDNLSENNWAEAATGIMTTDTLPKGASRQFQHDGVTVTVTGISKGA GMIRPNMATMLGYIATDAKVAPKVLKDLMLDGANKSFNRITIDGDTSTNDCCMLIATGKA DLPEVTEASGALFEALKKAVFEVCMEVAQAIVRDGEGATKFVTVQVNGGGNHQECLDVGY AVAHSPLIKTALFASDPNWGRILAAVGRAGVPELDVSLIDVYLDSVCIASKGGRSPSYTE AQGSAVMAQEEITIRIELGRGQCSETIWTTDLSHEYVKINAEYRT