Protein Info for PP_1345 in Pseudomonas putida KT2440

Annotation: protein translocation complex, ATPase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 939 PF07517: SecA_DEAD" amino acids 34 to 430 (397 residues), 432.9 bits, see alignment E=1.7e-133 TIGR00963: preprotein translocase, SecA subunit" amino acids 56 to 846 (791 residues), 1106.1 bits, see alignment E=0 PF00270: DEAD" amino acids 124 to 244 (121 residues), 25.9 bits, see alignment E=2.3e-09 PF01043: SecA_PP_bind" amino acids 260 to 386 (127 residues), 118.5 bits, see alignment E=6.4e-38 PF21090: P-loop_SecA" amino acids 445 to 642 (198 residues), 305 bits, see alignment E=7.6e-95 PF07516: SecA_SW" amino acids 645 to 858 (214 residues), 243.1 bits, see alignment E=8.8e-76 PF02810: SEC-C" amino acids 919 to 936 (18 residues), 41 bits, see alignment (E = 4.1e-14)

Best Hits

Swiss-Prot: 83% identical to SECA_AZOVD: Protein translocase subunit SecA (secA) from Azotobacter vinelandii (strain DJ / ATCC BAA-1303)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 83% identity to avn:Avin_13340)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N69 at UniProt or InterPro

Protein Sequence (939 amino acids)

>PP_1345 protein translocation complex, ATPase subunit SecA (Pseudomonas putida KT2440)
MLVRMRPMGCTGDSWGRPPSLSVEDPADMFAPLLKKLFGSKNEREVKRMLKTVSIVNAFE
EKMVALSDEQLRGKTAEFKERLAKGETLDQLLPEAFAVAREAGKRVMGMRHFDVQLIGGM
TLHEGMIAEMRTGEGKTLVGTLAVYLNALSGKGVHVVTVNDYLARRDANWMRPLYEFLGL
SVGIVSAFQPPEEKRAAYAADITYGTNNEFGFDYLRDNMAFSQDEKFQRELNFAVIDEVD
SILIDEARTPLIISGQAEDSSKLYIEINRLIPRLTQHIEEVEGQVTQEGHFTIDEKSRQV
ELNEAGHQFIEEMLAQAGLLAEGESLYSAHNLGLLTHVYAGLRAHKLFHRNVEYIVQDGQ
VLLIDEHTGRTMPGRRLSEGLHQAIEAKENLNIQAESQTLASTTFQNYFRLYTKLSGMTG
TADTEAFEFQSIYGLNVMVIPPNKPLARKDYNDLVYLTADEKYAAIIADIKESMKLGRPV
LVGTATIETSEHMSNLLKKEGIDHKVLNAKYHEKEAEIIAQAGAPGALTIATNMAGRGTD
ILLGGNWEAEVAALENPTAEQIAQIKADWQKRHQQVIETGGLHVIASERHESRRIDNQLR
GRSGRQGDPGSSRFYLSLEDSLMRIFASDRVKNFMKALGMQSGEAIEHRMVTNAIEKAQR
KVEGRNFDIRKQLLEYDDVANEQRKVIYHMRNSLLAAENIGDTIVEFRKEVLDATISQHI
PPQSLPEQWDVAGLEASLASDFAIKLPIRQWLDEDDHLYEETLREKLLSEITTAYTEKED
QAGLEALRTFEKQILLRVLDDLWKDHLSTMDHLRHGIHLRGYAQKNPKQEYKRESFSLFQ
ELLESIKRDTIRVLSHVQVRREDPAEEEARLRREAEELASRMQFQHAAAPGLGSEQLSEE
GAEVAVASAPVRNDQKLGRNEPCWCGSGKKFKHCHGQIE