Protein Info for PP_1337 in Pseudomonas putida KT2440

Annotation: UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 TIGR01133: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase" amino acids 4 to 352 (349 residues), 360.7 bits, see alignment E=3.7e-112 PF03033: Glyco_transf_28" amino acids 8 to 143 (136 residues), 143.3 bits, see alignment E=8.2e-46 PF13579: Glyco_trans_4_4" amino acids 22 to 141 (120 residues), 32.2 bits, see alignment E=1.9e-11 PF04101: Glyco_tran_28_C" amino acids 185 to 337 (153 residues), 121.5 bits, see alignment E=5.9e-39

Best Hits

Swiss-Prot: 100% identical to MURG_PSEPK: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K02563, UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase [EC: 2.4.1.227] (inferred from 100% identity to ppu:PP_1337)

MetaCyc: 45% identical to N-acetylglucosaminyl transferase (Escherichia coli K-12 substr. MG1655)
acetylglucosaminyltransferase. [EC: 2.4.1.227]

Predicted SEED Role

"UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227)" in subsystem Peptidoglycan Biosynthesis (EC 2.4.1.227)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.227

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N76 at UniProt or InterPro

Protein Sequence (359 amino acids)

>PP_1337 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (Pseudomonas putida KT2440)
MAAEGKNVLIMAGGTGGHVFPALACAREFQARGYSVHWLGTPRGIENELVPQAGLPLHLI
QVSGLRGKGKLSLLKAPFTLVKAVLQARRIIRQLKPVCVLGFGGYVTGPGGVAARLCGVP
LVIHEQNARAGTANRLLVPLSARVCEAFPNTFEASDKRRTTGNPVRPELFMDAQRTPLGE
RRARLLVLGGSLGAEPLNKLLPKALSEVPAALRPEVFHQAGKQHAPITAERYHEAGVAAQ
VEPFIKDMAQAYGWADLVVCRAGALTVSELAAAGLPSMLVPLPHAIDDHQTHNAQYLARE
GAAFLMPQATTGAAQLAERLNEVLMQPEKLNVMAGTARRLAKPAATSTVVDICLEVAHG