Protein Info for PP_1334 in Pseudomonas putida KT2440

Annotation: Phospho-N-acetylmuramoyl-pentapeptide- transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 72 to 91 (20 residues), see Phobius details amino acids 97 to 114 (18 residues), see Phobius details amino acids 134 to 152 (19 residues), see Phobius details amino acids 168 to 189 (22 residues), see Phobius details amino acids 199 to 220 (22 residues), see Phobius details amino acids 233 to 256 (24 residues), see Phobius details amino acids 263 to 284 (22 residues), see Phobius details amino acids 289 to 311 (23 residues), see Phobius details amino acids 338 to 357 (20 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 38 to 360 (323 residues), 466.5 bits, see alignment E=2.6e-144 PF10555: MraY_sig1" amino acids 68 to 80 (13 residues), 20.3 bits, see alignment (E = 2.9e-08) PF00953: Glycos_transf_4" amino acids 99 to 284 (186 residues), 121.2 bits, see alignment E=4.3e-39

Best Hits

Swiss-Prot: 100% identical to MRAY_PSEPK: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 100% identity to ppu:PP_1334)

MetaCyc: 64% identical to phospho-N-acetylmuramoyl-pentapeptide-transferase (Escherichia coli K-12 substr. MG1655)
Phospho-N-acetylmuramoyl-pentapeptide-transferase. [EC: 2.7.8.13]

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N79 at UniProt or InterPro

Protein Sequence (360 amino acids)

>PP_1334 Phospho-N-acetylmuramoyl-pentapeptide- transferase (Pseudomonas putida KT2440)
MLLLLAEYLQQFHKGFAVFQYLSLRGILGVLTALSLALWLGPWMIRTLQIRQIGQAVRND
GPQSHLSKSGTPTMGGALILSAIAVSTLLWADLSNRYVWVVLIVTLAFGAIGWVDDYRKV
IEKNSRGLPSRWKYFWQSVFGLAAAVFLYKTAPTSVETTLILPFIKDVTIPLGVGFVVLT
YFVIVGSSNAVNLTDGLDGLAIMPTVMVGGALGIFCYLSGNVKFAEYLLIPYVPGSGELI
VFCGALIGAGLGFLWFNTYPAQVFMGDVGALALGAALGTIAVIVRQEIVLFIMGGIFVVE
TLSVVIQVASFKLTGKRVFRMAPIHHHFELKGWPEPRVIVRFWIITVILVLIGLATLKLR