Protein Info for PP_1332 in Pseudomonas putida KT2440

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 transmembrane" amino acids 304 to 323 (20 residues), see Phobius details TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 19 to 488 (470 residues), 520.8 bits, see alignment E=1.8e-160 PF01225: Mur_ligase" amino acids 19 to 95 (77 residues), 44.5 bits, see alignment E=2.5e-15 PF08245: Mur_ligase_M" amino acids 107 to 315 (209 residues), 172.2 bits, see alignment E=2.1e-54 PF02875: Mur_ligase_C" amino acids 338 to 464 (127 residues), 120.3 bits, see alignment E=1.5e-38

Best Hits

Swiss-Prot: 100% identical to MURE_PSEPK: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 100% identity to ppu:PP_1332)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88N81 at UniProt or InterPro

Protein Sequence (496 amino acids)

>PP_1332 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase (Pseudomonas putida KT2440)
MMTMPLSKLFAHASRDPLIRELTLDSRSVRPGDLFLAVPGAKVDGREHIADALARGAAAV
AYEEQGANVLPLTDVPLIPVKGLIAQLSDIAGRFYGEPSRQLNLVGVTGTNGKTSVTQLV
AQALDLLGQRCGLIGTLGTGFYGELQSGRLTTPDPIAVQSTLNDLKKGGARAVAMEVSSH
ALEQGRVAALEFDIAVMTNLSRDHLDYHGSMEAYEAAKAKLFAWPSLRCQVVNLDDDFGR
RLAADFARRPSVDHIETRLLSYSLESPEASLFCREAVFSDDGVRATLVTAQGERILRSQL
LGRFNLSNMLAAVATLLALDYALDEILKVTPQLQGPVGRMQRLGGGDKPLVVVDYAHTPD
ALEKVLEALRPHAHGKLLCLFGCGGDRDRGKRPLMAEVAERLADRVLVTDDNPRTEDPSR
IFDDIRPGFTRPDDVEFVAGRGEAIAHLIATAAANDVIVLAGKGHEDYQEINGERHDFSD
LTEAEKALAAWEAPHA