Protein Info for PP_1299 in Pseudomonas putida KT2440

Annotation: putative amino acid ABC transporter - membrane subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 transmembrane" amino acids 30 to 51 (22 residues), see Phobius details amino acids 96 to 115 (20 residues), see Phobius details amino acids 122 to 143 (22 residues), see Phobius details amino acids 157 to 181 (25 residues), see Phobius details amino acids 199 to 220 (22 residues), see Phobius details amino acids 233 to 250 (18 residues), see Phobius details amino acids 271 to 313 (43 residues), see Phobius details amino acids 326 to 351 (26 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 155 to 251 (97 residues), 69.2 bits, see alignment E=1.8e-23 PF00528: BPD_transp_1" amino acids 172 to 359 (188 residues), 82.7 bits, see alignment E=1.4e-27

Best Hits

Swiss-Prot: 66% identical to YHDY_ECOLI: Inner membrane amino-acid ABC transporter permease protein YhdY (yhdY) from Escherichia coli (strain K12)

KEGG orthology group: K09971, general L-amino acid transport system permease protein (inferred from 99% identity to ppg:PputGB1_4550)

Predicted SEED Role

"Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NB3 at UniProt or InterPro

Protein Sequence (365 amino acids)

>PP_1299 putative amino acid ABC transporter - membrane subunit (Pseudomonas putida KT2440)
MNAHVFKPDMPPPVKTVGVLAWMRANLFSSWLNTLLTLFAIYLVWLIVPPLLQWALIDAN
WVGTTRADCTKEGACWVFVQQRFGQFMYGYYPTELRWRVDLTVWLAVLGAAPLFIKRFPR
KAFYGLGFLVLYPVLAYTLLHGGYLGLESVPTSQWGGLMLTLVIATVGIVGALPLGILLA
LGRRSRMPAVKVVCVTFIEFWRGVPLITVLFMSSVMLPLFLPEGMSFDKLLRAMIGVILF
QSAYIAEVVRGGLQAIPKGQYEAAAAMGLGYWRSMGLVILPQALKLVIPGIVNTFIALFK
DTSLVIIIGLFDLLNSVKQAAADPAWLGMATEGYVFAALVFWIFCFGMSRYSMHLERKLD
TGHKR