Protein Info for PP_1296 in Pseudomonas putida KT2440

Annotation: Carboxylesterase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 218 PF02230: Abhydrolase_2" amino acids 3 to 215 (213 residues), 154.1 bits, see alignment E=7.4e-49 PF12697: Abhydrolase_6" amino acids 17 to 152 (136 residues), 30.3 bits, see alignment E=9.9e-11

Best Hits

Swiss-Prot: 69% identical to EST2_PSEFL: Carboxylesterase 2 (estB) from Pseudomonas fluorescens

KEGG orthology group: K06999, (no description) (inferred from 100% identity to ppu:PP_1296)

Predicted SEED Role

"phospholipase/carboxylesterase family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NB6 at UniProt or InterPro

Protein Sequence (218 amino acids)

>PP_1296 Carboxylesterase 2 (Pseudomonas putida KT2440)
MTNPLILEPQKTADACVIWLHGLGADRYDFLPVAEFMQERLLSTRFIMPQAPTRPVTING
GYAMPSWYDIKAMTPARAIDEAQLEESAEQVVALIKAEQAKGIDLTRIFLAGFSQGGAVV
LHTAYIKWQEALGGVIALSTYAPTFNDQHQLSACQQRTPALCLHGVHDSVVIPSMGRTAF
EYLNTWGVAARWYEYPMEHEVVVEELNDIHDWLSKQLQ