Protein Info for PP_1288 in Pseudomonas putida KT2440

Annotation: GDP-mannose 6-dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF03721: UDPG_MGDP_dh_N" amino acids 1 to 189 (189 residues), 204.5 bits, see alignment E=1.7e-64 TIGR03026: nucleotide sugar dehydrogenase" amino acids 1 to 418 (418 residues), 455.2 bits, see alignment E=9.7e-141 PF00984: UDPG_MGDP_dh" amino acids 204 to 298 (95 residues), 99.3 bits, see alignment E=1.7e-32 PF03720: UDPG_MGDP_dh_C" amino acids 317 to 419 (103 residues), 56.4 bits, see alignment E=5.1e-19

Best Hits

Swiss-Prot: 100% identical to ALGD_PSEPK: GDP-mannose 6-dehydrogenase (algD) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00066, GDP-mannose 6-dehydrogenase [EC: 1.1.1.132] (inferred from 100% identity to ppf:Pput_4437)

MetaCyc: 78% identical to GDP-mannose 6-dehydrogenase (Pseudomonas aeruginosa)
GDP-mannose 6-dehydrogenase. [EC: 1.1.1.132]

Predicted SEED Role

"GDP-mannose 6-dehydrogenase (EC 1.1.1.132)" in subsystem Alginate metabolism (EC 1.1.1.132)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.132

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NC4 at UniProt or InterPro

Protein Sequence (438 amino acids)

>PP_1288 GDP-mannose 6-dehydrogenase (Pseudomonas putida KT2440)
MRISIFGLGYVGAVCAGCLTARGHEVIGVDVSSTKIDLINQGKSPIVEPGLEALLQQGIA
NGRLRGTTDFAEAIRASDVSMICVGTPSKKNGDLGLEYIESVCREIGYVLRDTTRRHTIV
VRSTVLPGTVKNVVIPILEDCSGKKAGVDFGVAVNPEFLRESTAIKDYDQPPMTVIGELD
SASGDILQALYEELDAPIIRKPIEVAEMIKYTCNVWHATKVTFANEIGNIAKAVGVDGRE
VMDVVCQDKVLNLSQYYMRPGFAFGGSCLPKDVRALTYRAASLDVRAPLLDSLMRSNESQ
VQNAFELIEAHDKRKVALLGLSFKAGTDDLRESPLVELAERLIGKGYQLDIYDENVQYAR
VHGANKDYIESKIPHVSSLLNANLQQVIDNADIIVLGNRDEQFRALALQAPAGKQVIDLV
GFMNKPTSTTARTEGICW