Protein Info for PP_1287 in Pseudomonas putida KT2440

Annotation: Glycosyltransferase alg8

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 51 to 73 (23 residues), see Phobius details amino acids 361 to 370 (10 residues), see Phobius details amino acids 374 to 418 (45 residues), see Phobius details amino acids 429 to 447 (19 residues), see Phobius details amino acids 475 to 495 (21 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 89 to 345 (257 residues), 151.6 bits, see alignment E=3.7e-48 PF13632: Glyco_trans_2_3" amino acids 186 to 417 (232 residues), 102.1 bits, see alignment E=3.6e-33

Best Hits

Swiss-Prot: 100% identical to ALG8_PSEPK: Glycosyltransferase alg8 (alg8) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_4438)

MetaCyc: 80% identical to mannuronosyl transferase catalytic subunit (Pseudomonas aeruginosa)
Alginate synthase. [EC: 2.4.1.33]

Predicted SEED Role

"Alginate biosynthesis protein Alg8" in subsystem Alginate metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NC5 at UniProt or InterPro

Protein Sequence (496 amino acids)

>PP_1287 Glycosyltransferase alg8 (Pseudomonas putida KT2440)
MLNMQRLQTVLLQCAGWLLYMSLLMLIALALPADIFDSQSKHFIFLVGAVGIWRYSMGAT
HFIRGMIFLYGVYPYLRRKVQKMGNATDPSHVYLMVTSFRIEALTTAQVYSSVIREAINC
GFPTTVVCSLVEMSDELLVKSLWAKYNPPAHVKLDIVRIAGTGKRDGLAYGFRAISRMLP
DENAVVAVIDGDTVLADGVVRKTVPWFKLFPNVGGLTTNEFCEVRGGYIMSEWHKLRFAQ
RHINMCSMALSKRVLTMTGRMSMFRASVVTNPEFIADVESDSLMHWRLGRFKFLTGDDKS
SWFSLMRLGYDTFYVPDAAINTVEHPPEKSFLKASRKLMYRWYGNNLRQNSRALGLGLRR
LGLFTSIVLFDQRVSMWTSLLGLTVAVIASLKFGMAFLLVYLLWIGITRLILTIMLLCSG
HNVGPAYPLILYYNQIIGALMKIYVFFRLDKQSWTRQPTALKRDLASFQQWFNTWSSRTM
TFSAASIFVAVLFMVV