Protein Info for PP_1285 in Pseudomonas putida KT2440

Annotation: Alginate biosynthesis protein AlgK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details PF08238: Sel1" amino acids 315 to 349 (35 residues), 22.7 bits, see alignment 5.7e-09 amino acids 352 to 385 (34 residues), 12.6 bits, see alignment (E = 9e-06)

Best Hits

Swiss-Prot: 100% identical to ALGK_PSEPK: Alginate biosynthesis protein AlgK (algK) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K07126, (no description) (inferred from 100% identity to ppu:PP_1285)

Predicted SEED Role

"Alginate biosynthesis protein AlgK precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NC7 at UniProt or InterPro

Protein Sequence (484 amino acids)

>PP_1285 Alginate biosynthesis protein AlgK (Pseudomonas putida KT2440)
MACEGRTMISDTYKVRVNLGLCALAAAITLAGCAGLPDQRLANEALKRGDTALAERNYKA
LADLGYSEAQVGLADIKVATRDPSQIKEAEATYRAAAATSPRAQARLGRLLVAKPDSTQA
EREEAETLLKQAAKQGQSNTLIPLAMLYLSYPQSFPKVNAQQQIDQWRAAGNPEAGLAQV
LLYRTQGTYDQHLGEVEKICKAALNTTDICYVELATVYQKRGQADQQAALLGQLKSAYAR
GAVPATRVDSVARVLADRSLGQTDEKTAKELLEQVAPANPASWVSLAQLVYDFPELGDTD
QLMAYIDKGREAEQPRAELLLGRLYYEGKTLPADAQKAEQHLQAAAEAGEISAHYYLGQL
YRRGYLGNVEPQKAVDHLLAAARGGQNSADYALAQLFSEGHGIRPQPGNAWVFAQLSQAN
PTPQSAELLQQLDQQLTPDQRNQAQQLLDQEKRARGSLAQGANSTLALEALQDDEKEVDG
EDSL