Protein Info for PP_1282 in Pseudomonas putida KT2440

Annotation: Alginate biosynthesis protein AlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF19040: SGNH" amino acids 58 to 192 (135 residues), 27.2 bits, see alignment E=4.7e-10 PF16822: ALGX" amino acids 62 to 327 (266 residues), 234.1 bits, see alignment E=3.8e-73 PF16824: CBM_26" amino acids 348 to 471 (124 residues), 209.1 bits, see alignment E=2.8e-66

Best Hits

Swiss-Prot: 100% identical to ALGX_PSEPK: Alginate biosynthesis protein AlgX (algX) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1282)

Predicted SEED Role

"Alginate biosynthesis protein AlgX" in subsystem Alginate metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88ND0 at UniProt or InterPro

Protein Sequence (479 amino acids)

>PP_1282 Alginate biosynthesis protein AlgX (Pseudomonas putida KT2440)
MTPHLMKLLGLSAALLAISQGVRAEDVKAPTFSAEPCCQLCPEAHDASRYTTRYQQNFTT
LVQAQGDWLFRTREDLRTEFNTTPAGYKRLQQVHDAFKKRGVELVVVYQPTRGLVNRNML
NPAEKAAFDYQKALGNYQAMLKRFASMGYNVPDLSPLTNEQLAAADQGKDFYFRGDQHWT
PYGAERAAKIVADTVHKMPAFEGIPRKEFETRKSGRMGKTGTLHNVAGQLCGTSYAVQYM
DQFATEPKGASGGDDLFGDSGNAQITLVGTSHSGKNYNFSGFLEQYIGADVLNVAFPGGG
LEGSMIQYLGSEEFQKNPPKILIWEFSPLYRLDQETIWRQILGLLDDGCDDRPALMSAST
TLKPGKNELMVNGKGGVIKDLINRNLQMDVKFEDPSVKVLQATLWYLNGRHEDIKLEKPE
TSDTDGRFVFQMREDEDWASQRLLAFEVQGPESGTQKVEAKLCKRNNFAVPAQTAQAGQ