Protein Info for PP_1272 in Pseudomonas putida KT2440

Annotation: putative Multidrug efflux MFS membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details PF00529: CusB_dom_1" amino acids 33 to 354 (322 residues), 35.8 bits, see alignment E=1.6e-12 PF16576: HlyD_D23" amino acids 58 to 302 (245 residues), 66 bits, see alignment E=7.7e-22 PF13533: Biotin_lipoyl_2" amino acids 62 to 106 (45 residues), 53.9 bits, see alignment 2.9e-18 PF13437: HlyD_3" amino acids 226 to 318 (93 residues), 52.3 bits, see alignment E=2.1e-17

Best Hits

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 100% identity to ppu:PP_1272)

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NE0 at UniProt or InterPro

Protein Sequence (367 amino acids)

>PP_1272 putative Multidrug efflux MFS membrane fusion protein (Pseudomonas putida KT2440)
MRWLEPWMISRTIEMTNNRKTLFIGSVLAVAVLAGIVGPWMFGSDHRQSTNDAYVTADYT
VVAPKVAGFIKQVLVEDNQQVTAGQLLATIDARDYQAALDAAQAQLLVAQAQSADARATL
ERQDALIAQAEAAVKAAQAEVAFADHEVNRYSRLAEQGAGTVQNAQQARSAVDQARARLA
NAQAARVAARKQVDILTAQVASADGQLKRAEAGLEKAQLDLSYTRITAPVDGMVGERALR
VGAYVNPGARLLSVVPLQQAYVVGNFQETQLTHVQPGQPVRISVDTFSGETLQGHVQSIA
PATGVTFAAVKPDNATGNFTKVVQRIPVKIVLDDGQPLLTRLRVGMSVEATIDTRGDKLD
GKEVSAR