Protein Info for PP_1261 in Pseudomonas putida KT2440

Annotation: 2-ketoaldonate reductase / hydroxypyruvate/glyoxylate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 5 to 318 (314 residues), 97.5 bits, E=8.1e-32
• PF02826: 2-Hacid_dh_C
  • amino acids 108 to 286 (179 residues), 199.1 bits, E=6e-63

Best Hits

Swiss-Prot: 50% identical to GHRB_YERE8: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 100% identity to ppu:PP_1261)

MetaCyc: 60% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem Glycolate, glyoxylate interconversions (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

• glucose degradation (oxidative) (5/5 steps found)
• L-arabinose degradation IV (4/8 steps found)
• ketogluconate metabolism (4/8 steps found)
• L-serine biosynthesis II (1/4 steps found)
• D-xylose degradation IV (3/7 steps found)
• formaldehyde assimilation I (serine pathway) (7/13 steps found)
• superpathway of pentose and pentitol degradation (10/42 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of type II polyketide products
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Butanoate metabolism
• C21-Steroid hormone metabolism
• Fructose and mannose metabolism
• Glutathione metabolism
• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Insect hormone biosynthesis
• Linoleic acid metabolism
• Nucleotide sugars metabolism
• Pentose phosphate pathway
• Polyketide sugar unit biosynthesis
• Pyruvate metabolism
• Retinol metabolism
• Tetrachloroethene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.43, 1.1.1.81

Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.26 or 1.1.1.43 or 1.1.1.79 or 1.1.1.81

Sequence Analysis Tools

See Q88NF1 at UniProt or InterPro

Protein Sequence (324 amino acids)

>PP_1261 2-ketoaldonate reductase / hydroxypyruvate/glyoxylate reductase (Pseudomonas putida KT2440)
MKKTVLAFSRITPAMAERLQQDFNVILPNPKLGDISAQFNEALPEAHGLIGVGRKLGRAQ
LEGAARLEVVSSVSVGYDNYDLDYFNERGIALTNTPDVLTESTADLGFSLIMGCARRTAE
LDAWTKAGNWQATVGPAHFGSDVHGKTLGIVGMGNIGAAVARRGRFGFNMPILYSGNSRK
TALEKELGAQFRSLDQLLAEADFVVIVVPLSDATRKLISSRELKLMKPSAFLINIARGPV
VDEAALIEALQAGTIRGTGLDVYEKEPLSDSPLFKLPNALTLPHIGSATAETREAMANRA
IDNLRAALLGERPRDLVNPQVWKG