Protein Info for PP_1229 in Pseudomonas putida KT2440

Annotation: putrescine permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 transmembrane" amino acids 20 to 45 (26 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 93 to 115 (23 residues), see Phobius details amino acids 130 to 146 (17 residues), see Phobius details amino acids 155 to 177 (23 residues), see Phobius details amino acids 197 to 216 (20 residues), see Phobius details amino acids 236 to 255 (20 residues), see Phobius details amino acids 279 to 302 (24 residues), see Phobius details amino acids 336 to 354 (19 residues), see Phobius details amino acids 360 to 382 (23 residues), see Phobius details amino acids 394 to 412 (19 residues), see Phobius details amino acids 418 to 436 (19 residues), see Phobius details PF00324: AA_permease" amino acids 21 to 406 (386 residues), 141.6 bits, see alignment E=3.2e-45 PF13520: AA_permease_2" amino acids 39 to 408 (370 residues), 100.4 bits, see alignment E=1.1e-32

Best Hits

Swiss-Prot: 62% identical to PUUP_ECOLI: Putrescine importer PuuP (puuP) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_1258)

MetaCyc: 62% identical to putrescine:H+ symporter PuuP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-69

Predicted SEED Role

"Putrescine importer"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NI0 at UniProt or InterPro

Protein Sequence (450 amino acids)

>PP_1229 putrescine permease (Pseudomonas putida KT2440)
MQPDHSASGNGQLRKTLRLWHVIIIGLAYLTPMTVFDTFGIVSGITAGHVPSAYILALAG
ILFTAVSYGTLVKRFPQSGSAYTYTQRAINPHVGFLVGWSSLLDYLLLPMVNALLAKLYL
SAMFPEVPEWMWVAGFVTLISLINMRSVNLVAHFNLLFVGVQVAIIAVFIYLCVRGLDQG
EGLGTTWSLIPFADSQTQFSALAAGATILCFSFLGFDAVTCLSEETRDPAKTIPRAIFLT
ALIGGVVFITVSYFIQAYFPTMARFHDQEAALPEIALYVGGKLFQSIFIACTVINTIASG
LASQTSVSRLLYVMGRDNVIPASVFARLHSRYKTPVLNIAVVGLISLSAIFFDLVTATSI
INFGALVAFSFVNLSVINHCYLREGNRKGLANQLKYLVLPTIGFCIIVSLWLDLNAHSLM
FGGIWAALGLVYLGWLTKAFRAAPPNYVAE