Protein Info for PP_1155 in Pseudomonas putida KT2440

Annotation: YaiC/YhcK/ArdA family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 transmembrane" amino acids 16 to 36 (21 residues), see Phobius details amino acids 42 to 62 (21 residues), see Phobius details amino acids 83 to 104 (22 residues), see Phobius details amino acids 116 to 136 (21 residues), see Phobius details amino acids 147 to 170 (24 residues), see Phobius details PF05230: MASE2" amino acids 14 to 102 (89 residues), 109.2 bits, see alignment E=8.7e-36 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 181 to 343 (163 residues), 168.4 bits, see alignment E=5.5e-54 PF00990: GGDEF" amino acids 185 to 340 (156 residues), 146.1 bits, see alignment E=8e-47

Best Hits

Swiss-Prot: 38% identical to DGCC_ECOLI: Probable diguanylate cyclase DgcC (dgcC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1155)

MetaCyc: 38% identical to diguanylate cyclase DgcC (Escherichia coli K-12 substr. MG1655)
Diguanylate kinase. [EC: 2.7.7.65]

Predicted SEED Role

"GGDEF domain protein"

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.65

Use Curated BLAST to search for 2.7.7.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NQ0 at UniProt or InterPro

Protein Sequence (357 amino acids)

>PP_1155 YaiC/YhcK/ArdA family protein (Pseudomonas putida KT2440)
MDNRNGKGLSFVKRIYLPRVIGLGIGLFTVMAAIAPLSPPSWAWLLVLFNGLLWPHVAYL
WAARSTTPYQAEQRNLLLDSLMGGFWTAAMHFNPLPSVTVLAMMTMNNVAAGGKRMVFRG
VLAQLAGILAAILLLGPGLQLTATPLQVYACLPMLGLYPLALGWVCYQLAIKLGDHKRRL
SALSLTDSLTGLFNHGAWKDLLQLKFQACKQQQGHAVIALIDIDHFKTINDTFGHVVGDC
VLRQLSAELRRSLREGDQAGRYGGDEFCVILPETSEAQACQAMERLRERVSSYRNPQLPH
LRISLSIGLSPFQASLESPEHWLEQADKALYIAKHAGRDQVNFARGEAATLRLAYPD