Protein Info for PP_1103 in Pseudomonas putida KT2440

Annotation: ATP-dependent helicase, DEAD box family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 TIGR04121: DEXH box helicase, DNA ligase-associated" amino acids 8 to 810 (803 residues), 1112.3 bits, see alignment E=0 PF00270: DEAD" amino acids 22 to 194 (173 residues), 94.7 bits, see alignment E=1.4e-30 PF04851: ResIII" amino acids 30 to 162 (133 residues), 30.4 bits, see alignment E=9e-11 PF00271: Helicase_C" amino acids 242 to 348 (107 residues), 43.4 bits, see alignment E=9.3e-15 PF19306: Lhr_WH" amino acids 390 to 538 (149 residues), 199 bits, see alignment E=9e-63 PF08494: DEAD_assoc" amino acids 596 to 781 (186 residues), 169.4 bits, see alignment E=2.2e-53

Best Hits

KEGG orthology group: K03724, ATP-dependent helicase Lhr and Lhr-like helicase [EC: 3.6.4.-] (inferred from 100% identity to ppu:PP_1103)

Predicted SEED Role

"FIG003033: Helicase domain protein"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NV1 at UniProt or InterPro

Protein Sequence (816 amino acids)

>PP_1103 ATP-dependent helicase, DEAD box family (Pseudomonas putida KT2440)
MPAATDLANAWFAKRGWKPFAFQRRVWAAVERGESGLLHASTGAGKTYAVWLAALRAFKP
RPQGRHLAPIQVLWVTPMRALAADTARALQAPLDELQLPWSVGVRSGDTNSAERARQARR
LPSVLITTPESLTLLLTRAKAREDFATLQLVVVDEWHELLGNKRGVQLQLALARLRHWHP
GLPTWGLSATLGNLQHARDVLLPQGGLLVQGRQDKALQVDTLLPKAIERFPWAGHMGLKM
LDQVCHEIDASASSLVFTNTRAQAEIWYQALLEARPDWAGLIALHHASLARDTRDWVERS
LKQGSLKAVICTSSLDLGVDFLPVERVLQIGSAKGIARLMQRAGRSGHAPGRRSRVTLVP
THSLELVEAAAARQALLAGHIEARFSPRLCMDVLVQHLVSMALGSGFRPEQLLAEVRSTW
AFRQLRDSQWQWALDFVCHGGSSLTAYPDYQRVERQADGVYRVTSERLARRHRMGIGTIV
SDANLQLKYWSKGGGGKTLGSVEEAFIARLRPGDTLVFAGRVLELVRVENMTAYVRRSTA
RKAAVARWNGGRMPLSSELADALVEQLDAAAHERFEGPEMRAVRPLLALQAQWSALPTTS
TLLAETFKSRQGWHLFLYPFAGRMANLGLANLIAWRVSRAQPLSVSIAVNDYGFELLSPA
QVDWASHLPQALGTADLLEDVLASLNAGEMALRRFREIAQIAGLVFGGYPAAQKSTRQIQ
ASSGLFYEVFRKHDAGNLLLGQARDEVLSEELEIERLHRQLLKMSELQLDLQALRRPGPL
AFALLVEGMRETLSTEKLADRIARMVAELEQAANAG