Protein Info for PP_1097 in Pseudomonas putida KT2440

Annotation: Methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 679 PF09334: tRNA-synt_1g" amino acids 7 to 395 (389 residues), 519.3 bits, see alignment E=1.4e-159 TIGR00398: methionine--tRNA ligase" amino acids 7 to 535 (529 residues), 584.9 bits, see alignment E=1.7e-179 PF00133: tRNA-synt_1" amino acids 12 to 73 (62 residues), 32.8 bits, see alignment 8.4e-12 PF19303: Anticodon_3" amino acids 411 to 518 (108 residues), 27.9 bits, see alignment E=4.9e-10 TIGR00399: methionine--tRNA ligase, beta subunit" amino acids 551 to 679 (129 residues), 140 bits, see alignment E=4.6e-45 PF01588: tRNA_bind" amino acids 583 to 677 (95 residues), 85.2 bits, see alignment E=7.1e-28

Best Hits

Swiss-Prot: 100% identical to SYM_PSEPK: Methionine--tRNA ligase (metG) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to ppu:PP_1097)

MetaCyc: 66% identical to methionine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Methionine--tRNA ligase. [EC: 6.1.1.10]; 6.1.1.10 [EC: 6.1.1.10]; RXN-23924 [EC: 6.1.1.10]

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88NV7 at UniProt or InterPro

Protein Sequence (679 amino acids)

>PP_1097 Methionine--tRNA ligase (Pseudomonas putida KT2440)
MSEPRQILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKLRGNQCIYVCADDAHGSAIML
RAEKEGITPEQLIANVQAEHSSDFADFLVDFDNFHSTHSDENRELSSLIYSRLREAGHIA
TRSVTQYFDPEKGMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYAPTELKNPKSAISGA
TPVLRDSQHFFFKLPDFQAMLQQWTRSGTLQDAVANKLAEWLDSGLQEWDISRDAPYFGF
EIPGEPGKYFYVWLDAPIGYMASFKNLCARRPELDFDAFWHEGSKAELYHFIGKDIVNFH
ALFWPAMLEGAGFRKPTAVNVHGYLTVNGAKMSKSRGTFIKARTYLDHLQPEYLRYYYAA
KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNEGVMVGGDAAPELTEAF
LAAAPSIAEAYEARDFGRAMREIMALADRANAWIADKAPWSLAKQEGKQDEVQAICAQGI
NLFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHLTRLENHQLNPFKALMSRIEPAKVE
AMVAASKEDLLAAEAKAPAGNGELAKDPLSAEIEFDTFAAVDLRVALIVKAEAVAGADKL
LQLTLDIGDERRNVFSGIKSAYPDPSKLEGRLTMMVANLKPRKMRFGVSEGMVMAAGPGG
EEIYLLSPDSGAKPGQRIK