Protein Info for PP_1037 in Pseudomonas putida KT2440

Annotation: Phosphoribosylformylglycinamidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1299 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1298 (1296 residues), 2137.4 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 148 (114 residues), 138.8 bits, see alignment E=1.6e-44 PF18072: FGAR-AT_linker" amino acids 169 to 218 (50 residues), 83.4 bits, see alignment (E = 2.6e-27) PF02769: AIRS_C" amino acids 430 to 587 (158 residues), 119.6 bits, see alignment E=2.9e-38 amino acids 839 to 969 (131 residues), 56.2 bits, see alignment E=9.2e-19 PF13507: GATase_5" amino acids 1043 to 1298 (256 residues), 364.7 bits, see alignment E=4.4e-113

Best Hits

Swiss-Prot: 67% identical to PUR4_HAHCH: Phosphoribosylformylglycinamidine synthase (purL) from Hahella chejuensis (strain KCTC 2396)

MetaCyc: 65% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P16 at UniProt or InterPro

Protein Sequence (1299 amino acids)

>PP_1037 Phosphoribosylformylglycinamidine synthase (Pseudomonas putida KT2440)
MLILRGAPALSAFRHGKLLEQLSQKVPAVTGLYAEFAHFADVDGELTADQQQVLGRLLKY
GPSVPVQEPTGRLFLVVPRLGTISPWASKASDIAHNCGLQSIQRLERGIAYYVAGTLSDA
DAELIAAELHDRMTQRVLGQLEQAADLFSHAQPKPMTSVDILAGGRDALAKANIDLGLAL
AEDEIDYLVNAFQGLKRNPNDIELMMFAQANSEHCRHKIFNASWDIDGQAQEKSLFGMIK
NTYQMHNEGVLSAYKDNASVIVGNVAGRFFPNPETRQYGAVQEPVHILMKVETHNHPTAI
APFSGASTGSGGEIRDEGATGRGAKPKAGLTGFTVSNLRIPGFEQPWEQAYGKPERIVDA
LDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQAINTPHGEEVRGYHKPIMLAGGMGNIRE
DHVQKGEITVGAKLIVLGGPAMLIGLGGGAASSVATGASSADLDFASVQRENPEMERRCQ
EVIDRCWQLGDENPIAFIHDVGAGGISNAFPELVNDGGRGGRFELRNVPNDEPGMAPHEI
WSNESQERYVLAVSAVDFERFKAICERERCPFAVVGEATEEQHLTVSDSHFGNTPVDMPL
DVLLGKPPRMHRSVTREAELGDDFDPSELDLDSAVQRVLNHPAVASKSFLITIGDRTITG
LVARDQMVGPWQVPVADCAVTATSFDVYTGEAMAMGERTPLALLDAPASGRMAIGETLTN
LAASRIEKLSDIKLSANWMSAAGHPGEDARLYDTVKAVGMELCPELGITIPVGKDSMSMK
TKWSDEGVEKSVTSPMSLIITGFAPVTDIRKTLTPQLRMDKGETDLILIDLGRGKNRMGA
SILAQTYGKIAAQAPDVDDAEDLKAFFAVIQGLNADGHLLAYHDRSDGGLVTTVLEMAFA
GHCGLDLQLDPLTDSKGEVPAILFNEELGAVIQVRQDATPDVLAQFSAAGLGEECVAVIG
KPVNNAEVTISLNGEVLFDDDRRMLQRQWAETSYQIQRLRDNADCADQEFDLLLEEDNPG
LSVKLGFDVNDDIAAPYIKKGVRPQVAILREQGVNGQVEMAAAFDRAGFAAIDVHMSDIL
AGRVDFEAFKGLVACGGFSYGDVLGAGEGWAKSALFNARARDAFQAFFERTDSFALGVCN
GCQMMSNLHELIPGTEYWPHFVRNRSEQFEARVAMVEVQKSNSIFLQGMAGSRMPIAIAH
GEGHAEFASEEALLEADVSGCVALRYVDNHGKVTEAYPANPNGSPRGITGLTSRDGRVTI
MMPHPERVFRAVQNSWRPDEWQEDAALMRMFRNARVWVN