Protein Info for PP_1031 in Pseudomonas putida KT2440

Annotation: IMP dehydrogenase and single strand DNA binding factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00478: IMPDH
  • amino acids 8 to 475 (468 residues), 556.7 bits, E=2.8e-171
• TIGR01302: inosine-5'-monophosphate dehydrogenase
  • amino acids 8 to 456 (449 residues), 634.7 bits, E=4.4e-195
• PF00571: CBS
  • amino acids 94 to 139 (46 residues), 38.6 bits, 1.7e-13
  • amino acids 148 to 203 (56 residues), 26.9 bits, 7.4e-10

Best Hits

Swiss-Prot: 73% identical to IMDH_ACICA: Inosine-5'-monophosphate dehydrogenase (guaB) from Acinetobacter calcoaceticus

KEGG orthology group: K00088, IMP dehydrogenase [EC: 1.1.1.205] (inferred from 100% identity to ppu:PP_1031)

MetaCyc: 68% identical to inosine 5'-monophosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
IMP dehydrogenase. [EC: 1.1.1.205]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• ureide biosynthesis (6/7 steps found)
• superpathway of purines degradation in plants (13/18 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.205

Sequence Analysis Tools

See Q88P22 at UniProt or InterPro

Protein Sequence (489 amino acids)

>PP_1031 IMP dehydrogenase and single strand DNA binding factor (Pseudomonas putida KT2440)
MLRISQEALTFDDILLVPGYSEVLPNEVSLKTRLTRGIELNIPLVSAAMDTVTEARLAIA
MAQEGGIGIIHKNMTIEQQAGEVRKVKKFEAGVVKDPITIDADATVRDLFELTRLNNISG
VPVLANGDLVGIVTSRDVRFENRLDAKVRDVMTPKERLVTVREGADKNEVRELLHKHRLE
KVLIVDDKFNLKGMMTVKDIEKAKAYPLASKDDQGRLRVGAAVGTGKDTGERVAALVAAG
VDVVVVDTAHGHSKGVIDRVRWVKETYPQVQVIGGNIATGAAAKALAEAGADAVKVGIGP
GSICTTRIVAGVGVPQISAIANVAAALEGTGVPLIADGGIRFSGDLSKAIVAGASCVMMG
SMFAGTEEAPGEVELFQGRSYKAYRGMGSLGAMAQAQGSSDRYFQDSSAGAEKLVPEGIE
GRVPYKGALAAIIHQLMGGLRSSMGYTGSATIEEMRTKPEFVRITGAGMAESHVHDVQIT
KEAPNYRVG