Protein Info for PP_1027 in Pseudomonas putida KT2440

Annotation: Exodeoxyribonuclease 7 large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 PF13742: tRNA_anti_2" amino acids 16 to 108 (93 residues), 102.2 bits, see alignment E=2.3e-33 TIGR00237: exodeoxyribonuclease VII, large subunit" amino acids 17 to 361 (345 residues), 436.4 bits, see alignment E=5.7e-135 PF01336: tRNA_anti-codon" amino acids 36 to 110 (75 residues), 50.9 bits, see alignment E=1.9e-17 PF02601: Exonuc_VII_L" amino acids 132 to 442 (311 residues), 379.4 bits, see alignment E=2.5e-117

Best Hits

Swiss-Prot: 100% identical to EX7L_PSEPK: Exodeoxyribonuclease 7 large subunit (xseA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03601, exodeoxyribonuclease VII large subunit [EC: 3.1.11.6] (inferred from 100% identity to ppu:PP_1027)

MetaCyc: 46% identical to exodeoxyribonuclease VII subunit XseA (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease VII. [EC: 3.1.11.6]

Predicted SEED Role

"Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)" in subsystem DNA repair, bacterial (EC 3.1.11.6)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.6

Use Curated BLAST to search for 3.1.11.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P26 at UniProt or InterPro

Protein Sequence (459 amino acids)

>PP_1027 Exodeoxyribonuclease 7 large subunit (Pseudomonas putida KT2440)
MIKDPFERLGLDREVLTVSQLNGRARVLLEDVFRSVWVEGEISNLARPASGHMYFTLKDS
GAQVRCALFRQNATRVRQALRDGLAVRVRGKVSLFEGRGDYQLILDTVEPAGDGALRLAF
EALKEKLGAEGLFSAERKKPLPAHPQRIGIITSPTGAVIRDIISVFGRRAPQVELNLIPT
AVQGREAIAQIVRAIRLADSLGFDALILARGGGSLEDLWCFNEEAVARAVAACVTPIVSA
VGHETDVSISDFVADVRAPTPSAAAELLAPDNSGLRQRLDGLQRRLLLRMQNRLTHDRLR
LESLTRRLRHPGERLRQRAQRLDDLDMRLRRAFMLNLNQRRERLARLDARLAAQHPGRNL
KLLNQRLDSLAERLPRAMREVLKDRRQRFQAQLQTLQVVSPLATLARGYSILLDEQGQAI
RSAEQTRNGQRLTARLNEGELLVRVEDNHLTPVTLSLLD