Protein Info for PP_1014 in Pseudomonas putida KT2440

Annotation: aldose-ketose isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 44% identical to SQUS_ECOLI: Sulfoquinovose isomerase (yihS) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_1014)

MetaCyc: 44% identical to sulfoquinovose isomerase (Escherichia coli K-12 substr. MG1655)
Mannose isomerase. [EC: 5.3.1.7]; RXN-15296 [EC: 5.3.1.7, 5.3.1.31]

Predicted SEED Role

"N-acylglucosamine 2-epimerase (EC 5.1.3.8)" in subsystem Sialic Acid Metabolism (EC 5.1.3.8)

MetaCyc Pathways

sulfoquinovose degradation III (2/4 steps found)
N-acetylneuraminate and N-acetylmannosamine degradation II (1/3 steps found)
sulfoquinovose degradation VI (2/5 steps found)
sulfoquinovose degradation I (1/5 steps found)
sulfoquinovose degradation V (1/7 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.8 or 5.3.1.31 or 5.3.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P39 at UniProt or InterPro

Protein Sequence (416 amino acids)

>PP_1014 aldose-ketose isomerase (Pseudomonas putida KT2440)
MTPLNLYVNSWLNAPAHTAWRLAEAQRLLAFAKAAKLPDGFGNLDANGQLAPGARAETIN
TARMTHCFALAHLQGIPGSLAYAEHGVAALRGAMQDATHGGWFAHPGGHDDSGKAAYLHA
FVALAASSAVVAGAVDANTLLADAIQVIEAHFWSEDEGALRETFSRAWQLPEQYRGANSN
MHATEAFLALADVTGNGLWLQRALRIAERIIHTHATANGYRVIEHFDVHWQPLPDYNLEH
PADPFRPYGTTPGHALEWARLLLHLEASLERAGLYAPQWLPDSARALFDTACQQAWNVDG
EPGFVYTLDWADRPVVHARLHWVHAEACAAAAALLQRTGEAHYEQWYRCCWGFIANHFID
PIGGSWHHELDAHNQPAGSLWPGKPDLYHAYQALLLPGLSLAPSLASNLAGNVTKR