Protein Info for PP_0997 in Pseudomonas putida KT2440

Annotation: Sigma-54 dependent transcriptional regulator/sensory box protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 PF13188: PAS_8" amino acids 81 to 133 (53 residues), 20.7 bits, see alignment 1e-07 PF00158: Sigma54_activat" amino acids 194 to 360 (167 residues), 225 bits, see alignment E=1.6e-70 PF14532: Sigma54_activ_2" amino acids 194 to 365 (172 residues), 73 bits, see alignment E=1e-23 PF07728: AAA_5" amino acids 217 to 336 (120 residues), 22.5 bits, see alignment E=3.4e-08 TIGR04381: TyrR family helix-turn-helix domain" amino acids 452 to 498 (47 residues), 87.6 bits, see alignment 2e-29 PF18024: HTH_50" amino acids 452 to 498 (47 residues), 52.1 bits, see alignment 1.4e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_1034)

Predicted SEED Role

"Sigma-54 dependent transcriptional regulator/sensory box protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P56 at UniProt or InterPro

Protein Sequence (502 amino acids)

>PP_0997 Sigma-54 dependent transcriptional regulator/sensory box protein (Pseudomonas putida KT2440)
MRIHVSFIDRVGITQEVLALLGARNLNLDAVEMVPPNVYIDAPTLSPAVLEELHDALFEV
HGVQSVDVVDILPGQRRHLQLDALLAAMSDPVLAVDSAGLVLLANPALIELCGRESAGRS
VGELFGDPALLQALLDNNFHLPMREMQLNGHSLLLDAMPITNAGGLLTLYPPSRMGERLS
ALHHDHAEGFDALLGESPAIRTLKARALRVAALDAPLLVHGETGTGKELVARACHAVSSR
HAAPFLALNCAALPESLAESELFGYAPGAFTGAQRGGKPGLMELANQGTVFLDEIGEMSP
YLQAKLLRFLSDGSFRRVGGDREVKVDVRIISATHRDLERMVAEGSFREDLFYRLNVLNL
EVPPLRDRGQDILMLAHFFMQQACTQIQRPACRLTPSTHSALLANPWPGNVRQLQNVIFR
AAAICEGNLVDIGDLDIAGTSVARGQDGEVASLEQAVGDFERELLQRLYASYPSTRQLAG
RLQTSHTAIAQRLRKYGIPGKP