Protein Info for PP_0980 in Pseudomonas putida KT2440

Annotation: Probable cytosol aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 PF02789: Peptidase_M17_N" amino acids 19 to 148 (130 residues), 123.1 bits, see alignment E=6.7e-40 PF00883: Peptidase_M17" amino acids 185 to 487 (303 residues), 440.2 bits, see alignment E=4.2e-136

Best Hits

Swiss-Prot: 100% identical to AMPA_PSEPK: Probable cytosol aminopeptidase (pepA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to ppu:PP_0980)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P73 at UniProt or InterPro

Protein Sequence (497 amino acids)

>PP_0980 Probable cytosol aminopeptidase (Pseudomonas putida KT2440)
MELVVKSVAAASVKTATLVLPVGENRKLGTVAQAVDQACEGAISAVLKRGDLAGKPGQTL
LLQNLSGLKAERVLLVGSGKEEALGDRAWRKLVASVAGVLKGLNGADAVLALDDIAVSNR
DAHYGKYRLLAETLLDGEYVFDRFKSQKAEPRALKKVTLLADKAGQAEVERAVKHASAIA
SGMAFTRDLGNLPPNLCHPSYLAEQAKELGKAHKALKVEVLDEKKIKDLGMGAFYAVGQG
SDQPPRLIVLNYQGGKKADKPFVLVGKGITFDTGGISLKPGAGMDEMKYDMCGAASVFGT
LRAVLELQLPINLVCLLACAENMPSGGATRPGDIVTTMSGQTVEILNTDAEGRLVLCDTL
TYAERFKPQAVIDIATLTGACIVALGSHTSGLMGNNDDLVGQLLDAGKRADDRAWQLPLF
EEYQEQLDSPFADMGNIGGPKAGTITAGCFLSRFAKAYNWAHMDIAGTAWVSGGKDKGAT
GRPVPLLTQYLLDRAGA