Protein Info for PP_0976 in Pseudomonas putida KT2440

Annotation: Ribosomal RNA large subunit methyltransferase F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 PF05971: Methyltransf_10" amino acids 5 to 302 (298 residues), 440.1 bits, see alignment E=4.3e-136 PF05175: MTS" amino acids 107 to 197 (91 residues), 28.6 bits, see alignment E=1e-10

Best Hits

Swiss-Prot: 100% identical to RLMF_PSEPK: Ribosomal RNA large subunit methyltransferase F (rlmF) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 100% identity to ppu:PP_0976)

MetaCyc: 52% identical to 23S rRNA m6A1618 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11596 [EC: 2.1.1.181]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P77 at UniProt or InterPro

Protein Sequence (317 amino acids)

>PP_0976 Ribosomal RNA large subunit methyltransferase F (Pseudomonas putida KT2440)
MTPNKPTLHPRNRHQGRYDFPSLIKAHPDLARFTITNPHGKPSIDFANPEAVRVFNRALL
KAQYGIQHWDIPADYLCPPIPGRADYIHVAADLLADDNAGDVPRGAQVRALDIGVGANCI
YPLLGYSDYRWRFLGSDIDPVALASAKAIVQANGLGKAITLRQQANRAHILSGLLQEGER
FDLTLCNPPFHASRDEATRGSQRKWKNLGKQDPKRKLPVLNFGGQNNELWCEGGEIRFVT
QLIGESVQYAERVLWFTSLVSKASNLPGIEAALKKSGAKAVRIIEMGQGQKQSRMVAWSF
LDDAARQAWHAQRKSQA