Protein Info for PP_0970 in Pseudomonas putida KT2440

Annotation: Cyanate MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 transmembrane" amino acids 49 to 67 (19 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 117 to 135 (19 residues), see Phobius details amino acids 141 to 162 (22 residues), see Phobius details amino acids 174 to 196 (23 residues), see Phobius details amino acids 203 to 223 (21 residues), see Phobius details amino acids 246 to 269 (24 residues), see Phobius details amino acids 283 to 304 (22 residues), see Phobius details amino acids 312 to 333 (22 residues), see Phobius details amino acids 339 to 360 (22 residues), see Phobius details amino acids 369 to 391 (23 residues), see Phobius details amino acids 402 to 421 (20 residues), see Phobius details TIGR00896: cyanate transporter" amino acids 51 to 401 (351 residues), 457.3 bits, see alignment E=1.7e-141 PF07690: MFS_1" amino acids 67 to 383 (317 residues), 74.6 bits, see alignment E=3.7e-25

Best Hits

Swiss-Prot: 32% identical to CYNX_ECOLI: Cyanate transport protein CynX (cynX) from Escherichia coli (strain K12)

KEGG orthology group: K03449, MFS transporter, CP family, cyanate transporter (inferred from 100% identity to ppu:PP_0970)

Predicted SEED Role

"Cyanate transport protein CynX" in subsystem Cyanate hydrolysis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88P83 at UniProt or InterPro

Protein Sequence (434 amino acids)

>PP_0970 Cyanate MFS transporter (Pseudomonas putida KT2440)
MNPETEKAMAESITRNSPSNERDLDELLIDAEADDEQVQQQAVVLRRPWLLLLGLVLVAL
NLRPALSSMAPVLGQVSEGLGLNASQAGLLTTLPVLCLGLFAPLAPVLARRFGSERVILG
ILLTLALGLVVRSTFGAIGVFLGSLMAGASIGIIGVLLPGIVKRDFPQHAGTLTGVYTMA
LCLGAAMAAGATVPLAQHFDGSWALGLGFWVLPALLAMLVWLPQARQGHGLHKVAYRVRG
LWRDPLAWQVTLYMGLQSSLAYIVFGWLPSILIGRGLTPTEAGLVLSGSVIVQLISSLSA
PWLATRGKDQRLAIVVVMSGTLAGLFGCLYAPLSGLWGWAIVLGLGQGGTFALALTLIVL
RSKDAHVAANLSSMAQGVGYTLASMGPFAVGLVHDLTGGWTALGWIFAVLGVGAVVFGLG
AGRALHVQVTCEKV