Protein Info for PP_0940 in Pseudomonas putida KT2440

Annotation: putative protease involved in Microcin B17 maturation and in sensitivity to the DNA gyrase inhibitor LetD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 PF01523: PmbA_TldD_1st" amino acids 38 to 101 (64 residues), 50 bits, see alignment E=4.1e-17 PF19290: PmbA_TldD_2nd" amino acids 127 to 235 (109 residues), 78.5 bits, see alignment E=8.1e-26 PF19289: PmbA_TldD_3rd" amino acids 243 to 476 (234 residues), 235.1 bits, see alignment E=7.9e-74

Best Hits

Swiss-Prot: 66% identical to TLDD_ECOLI: Metalloprotease TldD (tldD) from Escherichia coli (strain K12)

KEGG orthology group: K03568, TldD protein (inferred from 100% identity to ppg:PputGB1_0947)

MetaCyc: 66% identical to metalloprotease subunit TldD (Escherichia coli)
3.4.24.-

Predicted SEED Role

"TldD protein, part of TldE/TldD proteolytic complex"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PB0 at UniProt or InterPro

Protein Sequence (479 amino acids)

>PP_0940 putative protease involved in Microcin B17 maturation and in sensitivity to the DNA gyrase inhibitor LetD (Pseudomonas putida KT2440)
MSQMLSTVSEQLLAPGGLTLDSLQSVLGELAGPGIDAADLYFQGQISETWALEDGIVKEG
SFNLDQGVGVRAQSGEKTGFAYSNAINLEALTSAARAARSISRAGQNGTVQAFRSQEVAA
LYTADNPLDVLSRAEKVELLKRVDAATRALDPRIQQVSVSMAGVWERILIAAADGSLAAD
VRPLVRFNVSVIVEQNGRRERGGQGGGGRTDYRFFTEERVMGYAREALRQALVNLEAIPA
PAGTLPVVLGSGWSGVLLHEAVGHGLEGDFNRKGSSAFSGRIGEKVASSLCTIVDDGTLE
GRRGSLSVDDEGTPTECTTLIENGVLKGYMQDKLNARLMGMAVTGNGRRESYAHLPMPRM
TNTYMRAGESDPQEIIASVKKGIYCANLGGGQVDITSGKFVFSTSEAYLIEDGKITAPVK
GATLIGNGPEAMSRVSMVGNDLALDSGVGTCGKDGQSVPVGVGQPTLKLDAITVGGTGA