Protein Info for PP_0933 in Pseudomonas putida KT2440

Annotation: cell wall structural actin-like protein MreB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 TIGR00904: cell shape determining protein, MreB/Mrl family" amino acids 36 to 364 (329 residues), 534.4 bits, see alignment E=5.2e-165 PF06723: MreB_Mbl" amino acids 38 to 364 (327 residues), 492.5 bits, see alignment E=1.2e-151 PF00022: Actin" amino acids 59 to 229 (171 residues), 34.2 bits, see alignment E=2.9e-12 PF00012: HSP70" amino acids 133 to 234 (102 residues), 48.5 bits, see alignment E=1.1e-16 PF14450: FtsA" amino acids 187 to 345 (159 residues), 43.9 bits, see alignment E=7.4e-15

Best Hits

Swiss-Prot: 80% identical to MREB_ECOLI: Cell shape-determining protein MreB (mreB) from Escherichia coli (strain K12)

KEGG orthology group: K03569, rod shape-determining protein MreB and related proteins (inferred from 97% identity to pau:PA14_58150)

Predicted SEED Role

"Rod shape-determining protein MreB" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PB7 at UniProt or InterPro

Protein Sequence (372 amino acids)

>PP_0933 cell wall structural actin-like protein MreB (Pseudomonas putida KT2440)
MAPCPKSLPLLEFAALFYPRFPQGHFPMFKKLRGMFSSDLSIDLGTANTLIYVRERGIVL
NEPSVVAIRTHGNQKSVVAVGTEAKRMLGRTPGNIAAIRPMKDGVIADFSVCEKMLQYFI
NKVHENSFLQPSPRVLICVPCKSTQVERRAIRESALGAGAREVFLIEEPMAAAIGAGLPV
EEARGSMVVDIGGGTTEIALISLNGVVYAESVRVGGDRFDEAIVTYVRRNYGSLIGESTA
ERIKQEIGTAYPGGEVREVDVRGRNLAEGVPRAFTLNSNEVLEALQESLATIVQAVKSAL
EQSPPELASDIAERGLVLTGGGALLRDLDKLLAQETGLPVIVAEDPLTCVARGGGRALEM
MDKHAMDLLSSE