Protein Info for PP_0862 in Pseudomonas putida KT2440

Annotation: PKHD-type hydroxylase PP_0862

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 PF13640: 2OG-FeII_Oxy_3" amino acids 84 to 176 (93 residues), 55.5 bits, see alignment E=9.1e-19 PF18331: PKHD_C" amino acids 183 to 225 (43 residues), 81.3 bits, see alignment 4.1e-27

Best Hits

Swiss-Prot: 100% identical to Y862_PSEPK: PKHD-type hydroxylase PP_0862 (PP_0862) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K07336, PKHD-type hydroxylase [EC: 1.14.11.-] (inferred from 100% identity to ppu:PP_0862)

Predicted SEED Role

"Iron-uptake factor PiuC" in subsystem Transport of Iron

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.11.-

Use Curated BLAST to search for 1.14.11.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88PI8 at UniProt or InterPro

Protein Sequence (226 amino acids)

>PP_0862 PKHD-type hydroxylase PP_0862 (Pseudomonas putida KT2440)
MLLHIPGLFDADELARIREALERADWADGKVTAGYQSAKAKHNLQLPEGHALAKEIGTAL
IDRLWKTPRFMSAALPHKVFPPLINCYRDGGNFGFHIDNALRQPKGSPERVRTDLSSTLF
LSDPESYDGGELVIQDTYGVQQIKLAAGDMVLYPGTSLHKVNPVTRGQRYAAFFWTQSLV
RDDSQRALLFEMDNAIQQLTADVPDHPSLLQLTGTYHNLLRRWAEV